interleukin-8 and Arthritis--Reactive

This study was undertaken to evaluate the gene expression profile in monocytes from three patients with reactive arthritis (ReA) in remission in order to identify candidate genes accounting for a potential susceptibility to ReA. Gene expression analyses revealed eight differentially expressed mRNA transcripts in monocytes of ReA patients. The major part of genes encoded cytokines, growth factors and chemokines. There was a remarkably high proportion of proangiogenic factors, in particular IP10, ENA-78, and IL-8 accounting for a genetically determined susceptibility to ReA at the host cell level.

Topics: Arthritis, Reactive; Case-Control Studies; Chemokine CXCL5; Chlamydia Infections; Chlamydia trachomatis; Clostridioides difficile; Enterocolitis, Pseudomembranous; Gene Expression Profiling; Genetic Predisposition to Disease; Humans; Interleukin-8; Prohibitins; Receptors, Cytokine; RNA, Messenger; Yersinia enterocolitica; Yersinia Infections

We describe the cellular infiltrate and cytokine profile in sequential synovial membrane biopsies from a patient with acute followed by chronic synovitis after intravesical bacillus Calmette-Guérin (BCG) therapy for an in situ transitional cell carcinoma of the bladder. Histological and immunohistochemical analysis of 3 synovial biopsies were done sequentially over a 9 month period. The patient was HLA-B27 positive, but HLA-DR4 negative, and did not have the "shared epitope." Unlike other cases, this patient's arthritis did not respond initially to nonsteroidal antiinflammatory drugs and was exacerbated by corticosteroid therapy. The synovitis took a neutrophilic form, with marked synovial membrane content of interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-alpha). It subsequently developed into chronic lymphoplasmacytoid synovitis, similar to rheumatoid arthritis (RA), with decreased IL-8 but continuing IL-1 and TNF-alpha production in the synovial membrane. The synovitis resolved to a fibrotic synovium with residual thickening of the synovial lining layer and continued production of TNF-alpha. Thus, during the evolution of this arthritis, the synovial layer and continued production of TNF-alpha. Thus, during the evolution of this arthritis, the synovial membrane yielded a cellular infiltrate and cytokine content that had marked similarities with that seen in RA; however, the arthritis eventually remitted spontaneously.

Topics: Aged; Arthritis, Reactive; BCG Vaccine; Carcinoma, Transitional Cell; Cytokines; Female; HLA-B Antigens; HLA-DR Antigens; Humans; Immunohistochemistry; Interleukin-1; Interleukin-8; Synovial Membrane; Synovitis; Time Factors; Tumor Necrosis Factor-alpha; Urinary Bladder Neoplasms

Topics: Adult; Aged; Arthritis, Reactive; Cells, Cultured; Female; Humans; Interleukin-8; Male; Middle Aged; Monocytes; Yersinia Infections