interleukin-8 and Ankylosis

interleukin-8 has been researched along with Ankylosis* in 1 studies

Other Studies

1 other study(ies) available for interleukin-8 and Ankylosis

Article	Year
Interleukin-1beta stimulates interleukin-8 production and gene expression in synovial cells from human temporomandibular joint. Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, 2002, Volume: 60, Issue:7 The aims of the present study were to isolate and characterize cultured synovial cells from human temporomandibular joint (TMJ) specimens and to investigate the effect of interleukin (IL)-1beta on IL-8 production and gene expression in those cells.. Synovial cells (HTS cells) were isolated from TMJ synovial tissues using an outgrowth method and then primary cultured. The cells were examined for cell-specific markers of fibroblast, macrophage, and dendritic cells using immunocytochemistry. HTS cells were then treated with IL-1beta, and amounts of IL-8 were measured by enzyme-linked immunosorbent assay. IL-8 production and expression were also investigated using immunocytochemistry and a reverse transcription-polymerase chain reaction method.. HTS cells were positive for the fibroblast-specific markers, such as vimentin and propyl 4-hydroxylase. The macrophage or dendritic cell markers and HLA class II antigen were negative. Furthermore, IL-1beta enhanced IL-8 production in HTS cells in a time- and dose-dependent manner and stimulated IL-8 gene expression.. HTS cells may provide important advantages for studies of the cellular and molecular mechanisms in the TMJ. In addition, we found that IL-1beta stimulated IL-8 production through an increase in IL-8 gene expression in HTS cells, which may be associated with the increase of infiltrating inflammatory cells seen in the synovial membrane of TMJ disorders. Topics: Adult; Ankylosis; Antibodies, Monoclonal; Biomarkers; Cells, Cultured; Coloring Agents; Dendritic Cells; Dose-Response Relationship, Drug; Enzyme-Linked Immunosorbent Assay; Female; Fibroblasts; Gene Expression Regulation; Histocompatibility Antigens Class II; Humans; Immunohistochemistry; Interleukin-1; Interleukin-8; Joint Dislocations; Macrophages; Mixed Function Oxygenases; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Statistics as Topic; Synovial Membrane; Temporomandibular Joint; Temporomandibular Joint Disc; Temporomandibular Joint Disorders; Time Factors; Vimentin	2002

Article

Year

Interleukin-1beta stimulates interleukin-8 production and gene expression in synovial cells from human temporomandibular joint.

Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, 2002, Volume: 60, Issue:7

The aims of the present study were to isolate and characterize cultured synovial cells from human temporomandibular joint (TMJ) specimens and to investigate the effect of interleukin (IL)-1beta on IL-8 production and gene expression in those cells.. Synovial cells (HTS cells) were isolated from TMJ synovial tissues using an outgrowth method and then primary cultured. The cells were examined for cell-specific markers of fibroblast, macrophage, and dendritic cells using immunocytochemistry. HTS cells were then treated with IL-1beta, and amounts of IL-8 were measured by enzyme-linked immunosorbent assay. IL-8 production and expression were also investigated using immunocytochemistry and a reverse transcription-polymerase chain reaction method.. HTS cells were positive for the fibroblast-specific markers, such as vimentin and propyl 4-hydroxylase. The macrophage or dendritic cell markers and HLA class II antigen were negative. Furthermore, IL-1beta enhanced IL-8 production in HTS cells in a time- and dose-dependent manner and stimulated IL-8 gene expression.. HTS cells may provide important advantages for studies of the cellular and molecular mechanisms in the TMJ. In addition, we found that IL-1beta stimulated IL-8 production through an increase in IL-8 gene expression in HTS cells, which may be associated with the increase of infiltrating inflammatory cells seen in the synovial membrane of TMJ disorders.

Topics: Adult; Ankylosis; Antibodies, Monoclonal; Biomarkers; Cells, Cultured; Coloring Agents; Dendritic Cells; Dose-Response Relationship, Drug; Enzyme-Linked Immunosorbent Assay; Female; Fibroblasts; Gene Expression Regulation; Histocompatibility Antigens Class II; Humans; Immunohistochemistry; Interleukin-1; Interleukin-8; Joint Dislocations; Macrophages; Mixed Function Oxygenases; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Statistics as Topic; Synovial Membrane; Temporomandibular Joint; Temporomandibular Joint Disc; Temporomandibular Joint Disorders; Time Factors; Vimentin

2002