inositol-1-4-5-trisphosphate and Multiple-Organ-Failure

The purpose of the present study was to explore changes in rat colon motility, and determine the roles of calcium and inositol (1,4,5)-triphosphate (IP3) in colon dysmotility induced by multiple organ dysfunction syndrome (MODS) caused by bacteria peritonitis. The number of stools, the contractility of the muscle strips and the length of smooth muscle cells (SMC) in the colon, the concentration of calcium and IP3 in SMC, and serum nitric oxide were measured. Number of stools, fecal weight, IP3 concentration in SMC and serum nitric oxide concentration were 0.77 +/- 0.52 pellets, 2.51 +/- 0.39 g, 4.14 +/- 2.07 pmol/tube, and 113.95 +/- 37.89 micromol/L, respectively, for the MODS group (N = 11) vs 1.54 +/- 0.64 pellets, 4.32 +/- 0.57 g, 8.19 +/- 3.11 pmol/tube, and 37.42 +/- 19.56 micromol/L for the control group (N = 20; P < 0.05). After treatment with 0.1 mM acetylcholine and 0.1 M potassium chloride, the maximum contraction stress of smooth muscle strips, the length of SMC and the changes of calcium concentration were 593 +/- 81 and 458 +/- 69 g/cm(3), 48.1 +/- 11.8 and 69.2 +/- 15.7 microM, 250 +/- 70 and 167 +/- 48%, respectively, for the control group vs 321 +/- 53 and 284 +/- 56 g/cm(3), 65.1 +/- 18.5 and 87.2 +/- 23.7 microM, 127 +/- 35 and 112 +/- 35% for the MODS group (P < 0.05). Thus, colon contractility was decreased in MODS, a result possibly related to reduced calcium concentration and IP3 in SMC.

Topics: Animals; Calcium; Colon; Gastrointestinal Motility; Immunohistochemistry; Inositol 1,4,5-Trisphosphate; Male; Multiple Organ Failure; Myocytes, Smooth Muscle; Nitric Oxide; Rats; Rats, Wistar