inosinic-acid and Bone-Diseases

inosinic-acid has been researched along with Bone-Diseases* in 1 studies

Other Studies

1 other study(ies) available for inosinic-acid and Bone-Diseases

Article	Year
A new spectrophotometric method for the determination of 5'-nucleotidase. Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie, 1980, Volume: 18, Issue:11 A spectrophotometric method is described for the determination of 5'-nucleotidase. In combination with the enzymes nucleoside phosphorylase and xanthine oxidase, inosine, formed by hydrolysis of 5'-IMP by 5'-nucleotidase, is cleaved phosphorolytically to hypoxanthine, which is oxidized to uric acid. In the presence of ethanol, the hydrogen peroxide formed is reduced by catalase and equivalent amounts of acetaldehyde are produced. The aldehyde is dehydrogenated (NADP-dependent) by aldehyde dehydrogenase and the production rate of NADPH is recorded at 334 nm. The inhibition of the unspecific cleavage of 5'-IMP by phosphatases is examined critically. Topics: 5'-Nucleotidase; Aldehyde Oxidoreductases; Alkaline Phosphatase; Bone Diseases; Inosine Monophosphate; Liver Diseases; Nucleotidases; Phosphates; Purine-Nucleoside Phosphorylase; Spectrophotometry, Ultraviolet; Xanthine Oxidase	1980

Article

Year

A new spectrophotometric method for the determination of 5'-nucleotidase.

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie, 1980, Volume: 18, Issue:11

A spectrophotometric method is described for the determination of 5'-nucleotidase. In combination with the enzymes nucleoside phosphorylase and xanthine oxidase, inosine, formed by hydrolysis of 5'-IMP by 5'-nucleotidase, is cleaved phosphorolytically to hypoxanthine, which is oxidized to uric acid. In the presence of ethanol, the hydrogen peroxide formed is reduced by catalase and equivalent amounts of acetaldehyde are produced. The aldehyde is dehydrogenated (NADP-dependent) by aldehyde dehydrogenase and the production rate of NADPH is recorded at 334 nm. The inhibition of the unspecific cleavage of 5'-IMP by phosphatases is examined critically.

Topics: 5'-Nucleotidase; Aldehyde Oxidoreductases; Alkaline Phosphatase; Bone Diseases; Inosine Monophosphate; Liver Diseases; Nucleotidases; Phosphates; Purine-Nucleoside Phosphorylase; Spectrophotometry, Ultraviolet; Xanthine Oxidase

1980