inosine-pranobex and Fish-Diseases

Rhabdoviruses constitute one of the most pathogenic viruses isolated from rainbow trout and carp culture. Several viruses were also isolated from other species of fish. These viruses are mostly associated with epizootics and heavy losses. Spring viraemia of carp virus (SVCV) and pike fry rhabdovirus (PFRV) have been the most extensively studied, due to their significant economic impact. Significant progress has been made towards controlling the major bacterial fish diseases using vaccines, but this approach has not yet been successful in preventing viral diseases in fish culture. However, for an effective therapeutic approach, specific drugs should be developed to selectively inhibit virus replication and/or stimulate antiviral protection. In this investigation we examined the in vitro influence of methisoprinol on the SVCV and virus isolated from catfish (Ictalurus melas) replication by measuring their RNA synthesis. The viruses were propagated in EPC cells and cell cultures containing methisoprinol were followed by infection with SVCV or catfish rhabdovirus suspension containing 10(7) TCID50/ml. Methisoprinol (Polfa, Poland) at concentrations of 0, 100, 200, 300, 400 and 500 microg/ml of medium (Glasgow MEM) was used in this study. The results of this study show the strong inhibition of incorporation (cpm) of [3H]-uridine into SVCV and catfish rhabdovirus RNA in cell culture exposed to methisoprinol at various concentrations. The highest percent of inhibition of viral RNA at 72 h after infection with two rhabdoviruses were observed in doses of 400 and 500 microg/ml of methisoprinol in medium. The results of this in vitro study showed that methisoprinol inhibits the rhabdoviruses isolated from carp and catfish.

Topics: Animals; Carps; Cells, Cultured; Dose-Response Relationship, Drug; Fish Diseases; Ictaluridae; Inosine Pranobex; Rhabdoviridae; Rhabdoviridae Infections; RNA, Viral; Virus Replication

The aim of this study was to evaluate the in vitro influence of methisoprinol on head kidney phagocyte and lymphocyte activity after suppression induced by VHSV. The VHSV statistically significantly decreased the respiratory burst activity of head kidney phagocytes and the proliferative response of lymphocytes stimulated by ConA. The results of our study showed that methisoprinol at a concentration of 100 microl/ml modulated and restored the phagocyte and lymphocyte activity suppressed by VHSV.

Topics: Adjuvants, Immunologic; Animals; Fish Diseases; Inosine Pranobex; Kidney; Lymphocytes; Novirhabdovirus; Oncorhynchus mykiss; Phagocytosis; Rhabdoviridae Infections