inosine-diphosphate and Reperfusion-Injury

The influence of metabolic drug Cytoflavin (CF) with antihypoxic and antioxidative properties on human dermal fibroblasts in a model of ischemia-reoxygenation in vitro was studied. It was revealed that the restoration of ATP synthesis in fibroblasts in the postischemic period was considerably accelerated (in 2.1 times) by the addition of CF to the culture medium. The drug had a cell protective effect of reducing cell mortality during the reoxygenation after ischemia by 2-2.7 times. CF effectively reduced the level of reactive oxygen species (ROS) in fibroblasts after H2O2 treatment which allowed maintaining their survival at the level of control cells. Pretreatment of the cells with CF for one day ensured the maintenance of normal levels of ROS during the investigated time period in the fibroblasts subjected to H2O2 treatment, and reduced H2O2-induced cell death by almost a third compared to control cells. The introduction of CF in culture medium after ischemia showed no influence on Hsp70 synthesis, but led to decrease in GRP78 synthesis, raised after ischemia, to the control level, indicating a resolve of the endoplasmic reticulum (ER) stress and functional normalization of ER.

Topics: Adenosine Triphosphate; Antioxidants; Blotting, Western; Cell Culture Techniques; Cell Cycle; Cell Proliferation; Cell Survival; Cells, Cultured; Culture Media, Serum-Free; Data Interpretation, Statistical; Drug Combinations; Electrophoresis, Polyacrylamide Gel; Endoplasmic Reticulum Chaperone BiP; Energy Metabolism; Fibroblasts; Flavin Mononucleotide; Flow Cytometry; Heat-Shock Proteins; HSP70 Heat-Shock Proteins; Humans; Hydrogen Peroxide; Inosine Diphosphate; Models, Biological; Niacinamide; Oxidative Stress; Oxygen; Reactive Oxygen Species; Reperfusion Injury; Skin; Succinates

Topics: Aged; Brain; Carotid Arteries; Carotid Stenosis; Drug Combinations; Endarterectomy, Carotid; Female; Flavin Mononucleotide; Heme; Humans; Hypoxia-Ischemia, Brain; Inosine Diphosphate; Male; Middle Aged; Neuroprotective Agents; Niacinamide; Reperfusion Injury; Succinates

Experiment carried out on laboratory animals (rats) were aimed at comparative evaluation of the effect of several neuroprotective drugs under the conditions of model brain ischemia-reperfusion. The experimental methods included staining of brain tissue sections by hematoxiline-eosine, Nissl staining, and expression of NOS1, NOS3, TRAIL by imunnohistological means. The intensity of damage in various parts of brain and the nature of apoptosis without neuroprotection and with popular neuroprotectors (cytoflavin, actovegin, mexidol) and a test drug at the stage ofpreclinical trial (AKF-90-7) were evaluated. Characteristic cytotoxic (coagulative pycnomorphic and colliquative necrosis of neurons) and vascular (hemostasia, erythropedesis) changes were revealed. The neuroprotective effectof drugs decreases in the following order: AKF-90-7 > cytoflavin > actovegin > mexidol.

Topics: Animals; Brain; Drug Combinations; Drug Evaluation, Preclinical; Eosine Yellowish-(YS); Flavin Mononucleotide; Glycine; Hematoxylin; Heme; Hemostasis; Immunohistochemistry; Inosine Diphosphate; Male; Necrosis; Neurons; Neuroprotective Agents; Niacinamide; Nitric Oxide Synthase Type I; Nitric Oxide Synthase Type III; Picolines; Rats; Rats, Inbred Strains; Reperfusion Injury; Succinates; TNF-Related Apoptosis-Inducing Ligand

An analysis of experimental investigations carried out in 32 dogs and 30 rabbits and laboratory data of 242 patients has shown that the application of antioxidant and antihypoxic medicines decrease reperfusion lesions and endotoxicosis in operative treatment of acute intestinal obstruction.

Topics: Acute Disease; Animals; Digestive System Surgical Procedures; Disease Models, Animal; Dogs; Drug Combinations; Flavin Mononucleotide; Humans; Infusions, Intravenous; Inosine Diphosphate; Intestinal Obstruction; Intestine, Small; Male; Middle Aged; Niacinamide; Rabbits; Reperfusion Injury; Succinates; Treatment Outcome

Cytoflavin and neuronol produce vasoactive and neuroprotective effects in rats with cerebral ischemia. Vasoactive activity of neuronol was higher than that of cytoflavin. These differences were most pronounced at the level of microcirculation. Test preparations were equally potent in producing the neuroprotective effect. Cytoflavin and neuronol markedly decreased the mortality rate of animals. Over the first 6 h of ischemia the relative effectiveness of cytoflavin was higher than that of neuronol. However, neuronol exceeded cytoflavin in the relative effectiveness during the follow-up period (days 1-21).

Topics: Animals; Brain; Brain Ischemia; Cerebrovascular Circulation; Drug Combinations; Flavin Mononucleotide; Inosine Diphosphate; Male; Neuroprotective Agents; Niacinamide; Rats; Regional Blood Flow; Reperfusion Injury; Succinates; Succinic Acid; Survival Rate

Topics: Animals; Antioxidants; Drug Combinations; Energy Metabolism; Flavin Mononucleotide; Inosine Diphosphate; Lipid Peroxidation; Male; Meglumine; Neuroprotective Agents; Rats; Reperfusion Injury; Succinic Acid