indium-oxine and Uterine-Neoplasms

We present a case of urinary bladder fistulization which occurred approximately 25 yr after successful radiation therapy for uterine carcinoma. The extensive fistulas were detected with 111In-oxine-labeled white blood cell scintigraphy and were confirmed with a fistulogram, computed tomography and surgery. Scintigraphy was valuable for both initial detection as well as staging the extent of inflammation for subsequent diagnostic studies and surgical resection.

Topics: Cutaneous Fistula; Female; Humans; Indium Radioisotopes; Leukocytes; Middle Aged; Organometallic Compounds; Oxyquinoline; Radiation Injuries; Radionuclide Imaging; Time Factors; Urinary Bladder Fistula; Uterine Neoplasms

The homing characteristics and infiltrative capacity of interleukin-2 activated human peripheral blood lymphocytes, the lymphokine activated killer (LAK) cells, were studied. In vitro stimulated 111In-oxine labeled lymphocytes were injected into the hypogastric artery during hysterectomy, performed because of endometrial carcinoma. Scintigrams demonstrated clear homing of the lymphocytes into the area of the malignant tumor. No selective homing was detectable when labeled red blood cells were injected in a similar fashion. To analyze the infiltrative capacity of the activated lymphocytes, they were incubated in vitro with tumor spheroids grown from cultured glioma cell lines. As revealed by antibodies against the leukocyte common antigen and immunoperoxidase techniques, the activated lymphocytes infiltrated the three-dimensional tumor tissue slowly as a frontier. These results show that, in addition to their previously suggested potential role in cancer therapy, interleukin-2 activated lymphocytes may possibly also be useful as tumor tracers.

Topics: Female; Humans; Killer Cells, Lymphokine-Activated; Lymphocytes, Tumor-Infiltrating; Organometallic Compounds; Oxyquinoline; Radionuclide Imaging; Uterine Neoplasms