indigo-carmine and Peritoneal-Diseases

A 65-year-old male with end stage renal failure due to IgA nephritis commenced continuous ambulatory peritoneal dialysis (CAPD). Three weeks after initiation of CAPD, right hydrothorax developed. Then, we strongly assumed pleuroperitoneal communication( PPC) although the pleural effusion did not show high concentration of glucose. He underwent thoracoscopic surgery for PPC. On the inner, central tendons part of the diaphragm, there were a few blebs. One hour after 3 l of peritoneal dialysis solution containing 15 ml indigocarmine was instilled into the abdomen through a CAPD catheter, the blebs were tense and colored blue by the dye solution. The blebs were directly sutured and diaphragm was covered by polyglycolic acid sheet and fibrin glue all over. Since then, he resumed CAPD, without recurrence of hydrothorax. Then we investigated the treatment outcome of video-assisted thoracic surgery (VATS) for PPC in Japan. The outcome was poorer in cases in which communication was not detected intraoperatively. These patients should be given sufficient consideration for surgical procedure.

Topics: Aged; Coloring Agents; Humans; Hydrothorax; Indigo Carmine; Male; Peritoneal Dialysis, Continuous Ambulatory; Peritoneal Diseases; Pleural Diseases; Thoracoscopy

Topics: Coloring Agents; Contrast Media; Female; Fistula; Gastrectomy; Humans; Indigo Carmine; Iopamidol; Lymph Node Excision; Lymphatic Diseases; Lymphography; Middle Aged; Peritoneal Diseases

With the link between peritoneal adhesions and infertility well established, it is critical that materials used in pelvic surgery be tested for their adhesion-forming properties. The current study examined the adhesion-inducing properties of two dyes routinely used for visualization during pelvic surgery.. In vivo and in vitro examination of the effects of the dyes methylene blue and indigo carmine on adhesion formation in a mouse model.. A series of three experiments was conducted. In the first, dyes were injected directly into the peritoneal cavity. The mice were then sacrificed at one of two time points and the peritoneal cavity examined for adhesion formation. In addition, because of their purposed role in adhesion formation, macrophages from the cavity were examined for signs of dye-induced activation. Further studies of macrophage activation were then conducted in vitro to determine the effects of dye concentration and exposure time on the activation process.. Both methylene blue and indigo carmine appeared to induce adhesion formation as well as macrophage activation in vivo. Further, long-term exposure to visual concentrations of both dyes appeared to induce macrophage activation. However, only those macrophages exposed to methylene blue exhibited signs of activation when the exposure time was limited to times equivalent to those which might be expected during surgery.. Of the two dyes tested, indigo carmine might be the dye of choice in surgeries where fertility is to be maintained.

Topics: Abdomen; Animals; Indigo Carmine; Macrophage Activation; Methylene Blue; Mice; Mice, Inbred Strains; Microscopy, Phase-Contrast; Pelvis; Peritoneal Diseases; Random Allocation; Tissue Adhesions