indigo-carmine and Diseases-in-Twins

Since 1990 avoidance of methylene blue as a dye in diagnostic amniocentesis is recommended. This is a result of the observation that a high incidence of jejunoileal atresia appeared in twin pregnancies following intraamniotic injection of methylene blue. We report four further cases of jejunoileal atresia in twins after intraamniotic injection of dyes since 1991. We describe the clinical course, discuss possible teratogenic mechanisms and emphasize again that synthetic dyes should not be used in second trimester amniocentesis.

Topics: Abnormalities, Drug-Induced; Adult; Amniocentesis; Diseases in Twins; Female; Humans; Ileum; Indigo Carmine; Infant, Newborn; Intestinal Atresia; Jejunum; Male; Maternal Age; Methylene Blue; Pregnancy; Pregnancy, High-Risk; Pregnancy, Multiple; Twins

Indigo carmine is used commonly to mark the first-entered amniotic sac in twin amniocenteses. The presence of this dye significantly affects amniotic fluid bilirubin analysis. A method of chloroform extraction is recommended for determination of bilirubin in amniotic fluid previously contaminated by indigo carmine.

Topics: Amniocentesis; Amniotic Fluid; Anemia, Hemolytic; Bilirubin; Diseases in Twins; False Positive Reactions; Female; Fetal Diseases; Humans; Indigo Carmine; Indoles; Pregnancy

In contrast to former opinions detection of genetic disorders in early multiple pregnancy is possible by advanced techniques of amniocentesis Prenatal diagnosis has been performed in a twin pregnancy of an 41 years old patient by two ultrasonically guided amniocenteses. Injection of Indigo carmin after the first puncture showed two separate amnion cavities. Postpartal findings proved prenatal diagnosis.

Topics: Amniocentesis; Diseases in Twins; Female; Genetic Counseling; Humans; Indigo Carmine; Pregnancy; Pregnancy, Multiple; Ultrasonography