importazole and Sjogren-s-Syndrome

To examine genes expressed specifically in labial salivary glands (LSGs) of patients with Sjögren's syndrome (SS) in comparison with those of patients with immunoglobulin (Ig)G4-related disease (IgG4-RD), and to identify the genes involved in the pathogenesis of SS. Gene expression in LSGs of SS patients, IgG4-RD patients and healthy controls (HC) was analysed by cDNA microarray. Quantitative polymerase chain reaction (qPCR) was used to validate the up-regulation of differentially expressed genes (DEGs) in SS. Protein production of the validated gene in LSGs was examined by immunofluorescence (IF) assay. The association of molecular functions of the gene with the pathological conditions in SS was examined using peripheral blood lymphocytes. Among 1320 DEGs up-regulated in SS, qPCR confirmed the up-regulation of NR4A2 in LSGs of SS compared with IgG4-RD. IF staining showed higher production of NR4A2 in nuclei of CD4

Topics: Active Transport, Cell Nucleus; Adult; Aged; beta Karyopherins; Cell Differentiation; Cells, Cultured; DNA, Complementary; Female; Gene Expression Profiling; Humans; Immune System Diseases; Immunoglobulin G; Interleukins; Middle Aged; Nuclear Receptor Subfamily 4, Group A, Member 2; Quinazolines; Salivary Glands; Sjogren's Syndrome; Th17 Cells; Tissue Array Analysis