igk and Leukemia

Topics: B-Lymphocytes; Cell Line, Tumor; DNA Primers; Equipment Design; Gene Rearrangement; Genetic Techniques; Humans; Immunoglobulins; Leukemia; Lymphoma; Neoplasms; Polymerase Chain Reaction; Reagent Kits, Diagnostic; Sensitivity and Specificity; T-Lymphocytes

Classical t(11;14)(q13;q32) involving IGH-CCND1 is typically associated with aggressive CD5-positive mantle cell lymphoma (MCL). Recently, we identified the IGK variant of this translocation, t(2;11)(p11;q13), in three patients with a leukemic small-cell B-non-Hodgkin lymphoma. In all cases, rearrangements of the IGK and CCND1 genes were demonstrated by fluorescence in situ hybridization. Moreover, we mapped the 11q13 breakpoint of this variant translocation in the 3' region of CCND1 which contrasts with the 5' breakpoints in a standard t(11;14)(q13;q32). Expression of cyclin D1 was shown in two cases analyzed either at diagnosis or during disease progression. All three patients were asymptomatic at presentation and no initial therapy was required. One patient died of a progressive disease 58 months from diagnosis, and two patients showed stable disease after 12 months of follow-up. In two analyzed cases, mutated IGVH genes were identified. Our findings indicate that variant t(2;11)(p11;q13) does not typify a classical MCL but possibly a more indolent leukemic lymphoma originating from an antigen experienced (mutated) B cell.

Topics: Adult; Chromosomes, Human, Pair 11; Chromosomes, Human, Pair 2; Cyclin D1; Disease Progression; DNA, Neoplasm; Female; Gene Rearrangement, B-Lymphocyte, Heavy Chain; Genetic Variation; Humans; Immunoglobulin Heavy Chains; Immunoglobulin Variable Region; Immunoglobulins; In Situ Hybridization, Fluorescence; Karyotyping; Leukemia; Leukemia, Lymphocytic, Chronic, B-Cell; Lymphoma, Mantle-Cell; Lymphoma, Non-Hodgkin; Male; Middle Aged; Mutation; Neoplasm Recurrence, Local; Translocation, Genetic