i(3)so3-galactosylceramide and Neurilemmoma

Clonal cell line D6P2T, subcloned from an ethylnitrosourea-induced tumor line D6 of the rat peripheral nervous system, has been characterized with particular attention to galactolipid metabolism. Galactosylcerebroside and sulfatide synthesis and expression on the cell surface are highly regulated in D6P2T cells by mechanisms involving serum- and cyclic AMP-mediated pathways. These cells also express 2',3'-cyclic nucleotide 3'-phosphohydrolase (Wolfgram protein W1a) and laminin. In contrast, myelin basic protein and antigen HNK-1 were not detected. Line D6P2T appears to be a semi-differentiated Schwann cell model, which offers interesting possibilities for studies of galactolipid synthesis, transport, and sorting.

Topics: 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; 2',3'-Cyclic-Nucleotide Phosphodiesterases; Animals; Bucladesine; Cell Membrane; Chromatography, Thin Layer; Fluorescent Antibody Technique; Galactolipids; Galactose; Galactosylceramides; Glycolipids; Immunoassay; Laminin; Neurilemmoma; Peripheral Nervous System Neoplasms; Phosphoric Diester Hydrolases; Rats; Schwann Cells; Sulfates; Sulfoglycosphingolipids; Tumor Cells, Cultured

Several neural cell lines were examined for their ability to synthesize sulfatide and 2',3'-cyclic nucleotide phosphohydrolase, biochemical components characteristic of myelin. The mouse glioma G26 and the rat schwannoma TRM6B actively produced sulfatide, while the rat glioma C6 was inactive, supporting the probable oligodendroglial origin of the G26. In contrast, the C6 cell line had a high level of 2'-3'-cyclic nucleotide phosphohydrolase activity, while TRM6B showed 30% and the G26 75% lower activities. Thus, these two activities appear to be independently regulated.

Topics: 2',3'-Cyclic-Nucleotide Phosphodiesterases; Animals; Astrocytoma; Cell Line; Glioma; Mice; Myelin Sheath; Neoplasms, Experimental; Neurilemmoma; Neuroblastoma; Phosphoric Diester Hydrolases; Rats; Sulfoglycosphingolipids