i(3)so3-galactosylceramide and Hypothyroidism

Topics: Animals; Animals, Newborn; Brain Chemistry; Cerebrosides; Female; Fetus; Gangliosides; Growth Hormone; Hypothyroidism; Male; Pregnancy; Rats; Rats, Inbred Strains; Sex Factors; Sulfoglycosphingolipids

The action of neonatal hypothyroidism and early undernutrition on the lipid and protein composition, as well as, on the activity of 2'3' cyclic nucleotide 3' phosphohydrolase was studied in different subcellular fractions isolated from 20 day old hypothyroid and undernourished rats. Based on protein content, a marked decrease (70%) in the recovery of myelin was observed in both experimental conditions. The lipid composition of myelin in both groups was, however, different; while cholesterol, total phospholipids, and total galactolipids decreased in a similar fashion in the two situations; sulfatides and plasmalogens were much more affected in hypothyroid rats.

Topics: Animals; Animals, Newborn; Body Weight; Brain; Female; Gangliosides; Hypothyroidism; Lipid Metabolism; Male; Nerve Tissue Proteins; Nutrition Disorders; Organ Size; Phospholipids; Rats; Sulfoglycosphingolipids

Neonatal hypothyroidism reduced the concentration of sulfatide in myelin and synaptosomes isolated from brains of 20-21 day old rats. The in vivo incorporation of 35SO4 into sulfatide of myelin, microsomal, animals, as was the incorporation of the isotope into non-lipid sulfate (sulfated glycoproteins and mucopolysaccarides) of microsomal and synaptosomal fractions. In contrast, when animals were made hypothyroid at one month of age, no depletion of brain sulfatide or alteration of incorporation of 35SO4 into sulfatide was found, although incorporation of the isotope into non-lipid sulfate of myelin, mitochondria and synaptosomes was increased by this treatment. These results indicate that the metabolism of sulfatide and non-lipid sulfate of brain membranes is much more susceptible to alteration by hypothyroidism in neonatal than may alter the metabolims of sulfatide and non-lipid sulfate in neuronal as well as myelin membranes.

Topics: Aging; Animals; Animals, Newborn; Body Weight; Brain; Hypothyroidism; Male; Organ Size; Rats; Subcellular Fractions; Sulfates; Sulfoglycosphingolipids

Isolated terminal colon strips obtained from rats, made hypothyroid by methimazole treatment, were almost completely insensitive to the contractile effects of morphine as compared to strips from control animals. This low morphine sensitivity was accompanied by a significant (40%) reduction in the tissue sulfatide content. Although colon strips from hypothyroid rats showed a reduction of the contractile effects of acetylcholine, the decrease was considerably less than that noted for morphine. The addition of 6.6 X 10(-5) M tri-iodothyronine to colons from hypothyroid rats largely restored the tissue sensitivity to morphine. These results suggest that sulfatides could be linked to the in vitro effects of morphine in the colon.

Topics: Acetylcholine; Animals; Body Weight; Colon; Hypothyroidism; In Vitro Techniques; Male; Methimazole; Morphine; Muscle Contraction; Muscle, Smooth; Rats; Sulfoglycosphingolipids

Topics: Animals; Animals, Newborn; Body Weight; Brain; Brain Chemistry; Carbon Radioisotopes; Cerebrosides; Chromatography, Thin Layer; Fatty Acids; Galactose; Glycolipids; Hypothyroidism; Lipids; Methimazole; Organ Size; Rats; Sphingomyelins; Sulfoglycosphingolipids; Uridine Diphosphate Sugars

Topics: Animals; Animals, Newborn; Basal Ganglia; Brain; Brain Chemistry; Cerebrosides; Female; Hypothyroidism; Lipoproteins; Myelin Sheath; Nerve Tissue Proteins; Parathyroid Glands; Rats; Sulfoglycosphingolipids; Thyroid Gland