hyperoside and Depressive-Disorder--Major

Abelmoschus manihot (L.) Medic., a folk herbal medicine in China, is a flowering plant belonging\ to Abelmoschus L. genus and Malvaceae family, which has been reported with an antidepressant activity. The\ study was designed to isolate flavonoids from Abelmoschus manihot corolla and explore the action mechanism\ of antidepressant activities. The flavonoids were isolated and purified by D101 macroporous resin column,\ polyamide column and Sephadex LH-20 sequentially and identified as myricetin-3-O-β-D-glucoside (1),\ gossypetin-8-O-β-D-glucuronide (2, G-8-G), gossypetin-3'-O-β-D-glucoside (3), quercetin-3'-glucoside (4, Q-3-G),\ isoquercitrin (5, IQT), hyperoside (6, HY), myricetin (7), quercetin (8, QT). Compounds 2, 4, 5, 6 and 8\ (15, 30 and 60 mg·kg−1) were orally administered to mice and the reaction was observed in tail suspension\ test (TST) and forced swimming test (FST). Western blot analysis was used in determination of the protein\ expressions of brain-derived neurotrophic factor (BDNF), tyrosine receptor kinase B (TrkB) and phosphorylation\ eukaryotic elongation factor 2 (p-eEF2). The results revealed that only Q-3-G and G-8-G (15, 30, 60 mg ·kg−1)\ significantly reduced the immobility time in FST and TST. Furthermore, Q-3-G and G-8-G remarkably increased\ the expression of BDNF and TrkB, and decreased the expression of p-eEF2. These results suggest that\ Q-3-G and G-8-G had an obvious antidepressant activity via up-regulation of BDNF expression. The\ new observation will provide a new direction in the development of antidepressant in the treatment of major depressive\ disorder (MDD).

Topics: Abelmoschus; Animals; Antidepressive Agents; Brain-Derived Neurotrophic Factor; China; Depressive Disorder, Major; Drugs, Chinese Herbal; Ethanol; Flavonoids; Hindlimb Suspension; Hippocampus; Mice; Plant Extracts; Quercetin; Swimming; Up-Regulation