hypericum and Hemolysis

The top flowering aerial parts of the Hypericum species are traditionally used to prepare ointments to heal cuts and burns. Sometimes even the fruits are used for these purposes. Hypericum androsaemum L., commonly known as tutsan or shrubby St. John's Wort, is a Mediterranean medicinal plant which has been traditionally used to prepare an ointment for treating cuts and wounds.. To evaluate the extracts obtained from H. androsaemum red berries as functional ingredients for skin care formulations.. The methanolic extract was obtained by Soxhlet extraction while the aqueous extract was prepared by decoction; their composition was determined by HPLC analysis. Their biological activities were measured in terms of proliferation and migration of human fibroblasts, inhibition of collagenase activity, and immunomodulatory effects on human peripheral blood mononuclear cells (PBMCs). In addition, we evaluated their photostability by UV spectroscopy and their protective effects against APPH-induced hemolysis in red blood cells (RBC).. H. androsaemum red berries were proven to contain phytochemicals that improve skin regeneration, hence potentially employable in skin care formulations.

Topics: Adult; Cell Movement; Cells, Cultured; Chlorogenic Acid; Chromatography, High Pressure Liquid; Collagenases; Dermatologic Agents; Drug Stability; Female; Fibroblasts; Fruit; Hemolysis; Humans; Hypericum; Interleukin-6; Kinetics; Leukocytes, Mononuclear; Male; Matrix Metalloproteinase Inhibitors; Methanol; Middle Aged; Phytotherapy; Plant Extracts; Plants, Medicinal; Shikimic Acid; Skin Care; Solvents; Water; Wound Healing