hymecromone has been researched along with Thalassemia* in 1 studies
1 other study(ies) available for hymecromone and Thalassemia
Article | Year |
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A rapid and sensitive ELISA for serum ferritin employing a fluorogenic substrate.
A fluorescent enzyme-linked immunosorbent assay is described for the rapid measurement of serum ferritin. Increased sensitivity was achieved by using 4-methyl-umbelliferyl-beta-D-galactopyranoside as the substrate for beta-galactosidase coupled to the purified antiferritin antibody. Further enhancement of the specific antigen-antibody reaction was attained by the addition of 4% polyethylene glycol 6000 to the antiferritin-beta-galactosidase conjugate. The procedure is performed in microELISA plates. These modifications of the method permit the measurement of serum ferritin at concentrations ranging from 0.25 to 50 microgram/liter with a coefficient of variation of 8% or less. The entire procedure is performed at ambient temperature and is completed within one working day. The cost of the assay is less than 10% of the immunoradiometric assay for serum ferritin. Topics: Animals; beta-Galactosidase; Enzyme-Linked Immunosorbent Assay; Female; Ferritins; Galactosides; Humans; Hymecromone; Immune Sera; Male; Polyethylene Glycols; Rabbits; Spectrometry, Fluorescence; Spleen; Thalassemia | 1982 |