hydroxylysine and Scurvy

Topics: Animals; Ascorbic Acid; Carbon Isotopes; Cells, Cultured; Collagen; Culture Techniques; Dopamine beta-Hydroxylase; Granuloma; Guinea Pigs; Hydroxylation; Hydroxylysine; Hydroxyproline; Keto Acids; Lysine; Mixed Function Oxygenases; Oxidation-Reduction; Procollagen-Proline Dioxygenase; Proline; Protein Precursors; Scurvy; Tritium; Urine

Topics: Aging; Animals; Colchicine; Collagen; Collagen Diseases; Ehlers-Danlos Syndrome; Glucosyltransferases; Growth; Homocystinuria; Humans; Hydroxylysine; Hydroxyproline; Marfan Syndrome; Menkes Kinky Hair Syndrome; Penicillamine; Procollagen; Procollagen-Proline Dioxygenase; Protein Biosynthesis; Protein-Lysine 6-Oxidase; Scurvy; Skin; Transcription, Genetic; Vascular Diseases

Scorbutic guinea pigs were wounded and the influence of administering ascorbic acid 6 days later was studied with respect to cellular morphology, ribosomal distribution and protein synthesis. Electron-microscopic studies revealed that the dilated endoplasmic reticulum observed in the fibroblasts of scorbutic wound tissue had reverted to a normal configuration 24h after intraperitoneal injection of 100mg of ascorbate. Quantitative determination of the distribution of free and membrane-bound ribosomes indicated a significant increase in membrane-bound ribosomes in wound tissue from ascorbate-supplemented (recovery) animals. Sucrose-density-gradient centrifugation indicated a significant increase in the proportion of large membrane-bound polyribosomes in the range 300-350S and a concomitant decrease in 80S monoribosomes in the ribosome sedimentation profile of recovery tissue. Determination of the synthesis of non-diffusible [(3)H]hydroxyproline in scorbutic and recovery wounds showed a 3-4-fold stimulation in peptidyl-proline hydroxylation in recovery tissues. Studies carried out in which scorbutic and recovery tissues were incubated with [(14)C]leucine indicated that general protein synthesis, as measured by (14)C incorporated into non-diffusible material/mug of DNA, was unaltered by ascorbate supplementation. Similar studies of [(3)H]proline incorporation suggested that in recovery tissues there was a small but significant increase in [(3)H]proline incorporated/mug of DNA, which probably represents an increase in protocollagen synthesis. This observation correlates well with the increase seen in recovery tissues of large polyribosomes on which collagen precursor polypeptides are known to be synthesized. Preliminary characterization of the repair collagen synthesized by recovery animals showed it to be a typical Type I collagen having the chain composition (alpha(1))(2)alpha(2). The extent of glycosylation of the hydroxylysine of the newly synthesized collagen was greater than that reported for either normal guinea-pig dermal collagen or dermal scar collagen.

Topics: Animals; Ascorbic Acid; Centrifugation, Density Gradient; Collagen; Guinea Pigs; Hydroxylysine; Lysine; Male; Microscopy, Electron; Polyribosomes; Proline; Ribosomes; Scurvy; Skin; Wound Healing

Topics: Adult; Age Factors; Animals; Biopsy; Blood Coagulation Disorders; Blood Platelet Disorders; Collagen; Collagen Diseases; Female; Guinea Pigs; Humans; Hydroxylysine; Hydroxyproline; Lysine; Molecular Conformation; Platelet Adhesiveness; Proline; Scurvy; Skin; X-Ray Diffraction