hydroxylysine and Scleroderma--Systemic

Topics: Amino Acids; Blood Vessels; Cell Nucleus; Collagen; Connective Tissue; Cornea; Cytoplasm; Elasticity; Endothelium; Glycosaminoglycans; Humans; Hydroxylysine; Hydroxyproline; Proline; Regional Blood Flow; Rheology; Scleroderma, Localized; Scleroderma, Systemic; Skin; Skin Pigmentation; Sweat Glands; Ultrasonography

Topics: Collagen; Ehlers-Danlos Syndrome; Epidermolysis Bullosa; Glucocorticoids; Humans; Hydroxylysine; Hydroxyproline; Microbial Collagenase; Scleroderma, Systemic; Skin; Skin Diseases; Skin Neoplasms

Topics: Animals; Autoanalysis; Collagen; Colorimetry; Connective Tissue; Glycosaminoglycans; Humans; Hydroxylysine; Hydroxyproline; Methods; Microbial Collagenase; Organ Culture Techniques; Procollagen; Procollagen-Proline Dioxygenase; Scleroderma, Systemic

Small intestinal mucosa from 14 patients suffering from progressive systemic sclerosis and 22 patients with various other diseases was analysed for collagen components. There was no significant difference in the concentration of hydroxyproline, hydroxylysine and proline between the two groups. Rectal mucosa from 11 progressive systemic sclerosis patients, 5 patients with ulcerative colitis and 7 patients with various other diseases was analysed for collagen components. No significant difference was demonstrated in the concentration of hydroxyproline, hydroxylysine and proline between progressive systemic sclerosis patients and patients with various other diseases, but in patients with ulcerative colitis the concentration of hydroxyproline, hydroxylysine and proline were found to be significantly lower than in the two other groups.

Topics: Adolescent; Adult; Aged; Collagen; Duodenum; Female; Humans; Hydroxylysine; Hydroxyproline; Intestinal Mucosa; Male; Middle Aged; Proline; Rectum; Scleroderma, Systemic

The urinary excretion of uronic acid and the hydroxyproline of a urinary fraction of high molecular weight collagen peptides (% of total Hyp) indicate disease activity in generalized scleroderma. A significant decrease in the content of Hyp and Hyl in the skin of sclerodermal patients is not related to activity, but rather to the duration of the disease. Parallel analyses of skin and urine of the same patients are reported.

Topics: Adult; Aged; Connective Tissue; Female; Humans; Hydroxylysine; Hydroxyproline; Male; Middle Aged; Procollagen; Scleroderma, Systemic; Skin; Uronic Acids

In sclerodermal skin, the values for proline (Pro), hydroxyproline (Hyp) and hydroxylsine (Hyl) are lower than in normal skin. The molar ratio Hyp to Hyl is lowered. The molar ratio Pro to Hyp was found to be elevated, while that of Pro to Hyl was like that of normal skin. It was concluded that in slceroderma the hydroxylation of Pro to Hyp is incomplete, resulting in an abnormal collagen.

Topics: Collagen; Humans; Hydroxylation; Hydroxylysine; Hydroxyproline; Proline; Scleroderma, Systemic; Skin

Topics: Adolescent; Adult; Age Factors; Aged; Amino Acids; Animals; Biopsy; Collagen; Culture Techniques; Female; Humans; Hydroxylysine; Hydroxyproline; Male; Middle Aged; Norleucine; Penicillamine; Rats; Scleroderma, Localized; Scleroderma, Systemic; Skin