homoharringtonine and Cataract

To study the ability of homoharringtonine, 5-fluorouracil and adriamycin on inhibiting the proliferation of rabbit lens epithelial cells (RLEC) and the prevention of after cataract by using homoharringtonine.. RLEC were isolated and cultured. (1) The passage RLEC were placed in 24-well tissue culture plates and incubated for 48 hours, then exposed to different concentrations of homoharringtonine, 5-fluorouracil and adriamycin for 24 and 72 hours; (2) The passage RLEC and homoharringtonine, 5-fluorouracil, adriamycin were placed and cultured for 24 hours to investigate the rate of attached cells; (3) The morphological changes of RLEC were studied under light microscope.. The ID50 of homoharringtonine, 5-fluorouracil and adriamycin exposed to RLEC for 24 hours were 0.84 micrograms, 0.58 micrograms and 4.50 ng/ml and those for 72 hours were 0.49 micrograms, 0.33 micrograms and 3.85 ng/ml respectively. In the homoharringtonine group, the rate of attached cells was less than that of 5-fluorouracil and adriamycin groups. The study of the morphological changes showed that the different concentrations of antiproliferative drugs affected on RLEC at different regions.. The authors consider that homoharringtonine may be more effective for the prevention of after cataract than 5-fluorouracil and adriamycin.

Topics: Animals; Cataract; Cell Division; Cells, Cultured; Doxorubicin; Epithelial Cells; Fluorouracil; Growth Inhibitors; Harringtonines; Homoharringtonine; Lens, Crystalline; Rabbits

The cultures of human lens epithelial cells taken from both normal and cataractous lenses were established in vitro. The biological characteristics and the histological changes of the cells were investigated. 5-Fluorouracil (5-Fu), Homoharring tonine (HH) and Harmeline (H) were used for the inhibition of the proliferative cells in the experiment. The results revealed that the in vitro survival capacity of the human lens epithelial cells is limited and the proliferative capacity of the cells is negatively correlated to the donor age. HH and H can effectively inhibit the lens epithelial cell proliferation. Based on the results of this report, performing small and smoothly edged anterior capsulorhexis in extracapsular cataract extraction may possibly decrease the incidence of the post-operative opacification of the posterior capsule.

Topics: Adolescent; Adult; Age Factors; Cataract; Cell Division; Cells, Cultured; Child; Child, Preschool; Epithelial Cells; Epithelium; Fluorouracil; Harmaline; Harringtonines; Homoharringtonine; Humans; Infant; Lens Capsule, Crystalline; Postoperative Complications

To study the ability of Homoharringtonine (Hh), 5-Fluorouracil (5-Fu), and Adriamycin (ADM) on inhibiting the proliferation of rabbit lens epithelium.. Whole rabbit lenses were removed from freshly enucleated eyes under sterile condition. The rabbit lens epithelia (RLE) were isolated and cultured: (1) The passage RLE were placed in 24-well tissue culture plates and incubated for 48 hours, then exposed to different concentrations of Hh, 5-Fu, and ADM for 24 and 72 hours; (2) The passage RLE and Hh (0.084 microgram/ml), 5-Fu (0.058 microgram/ml), ADM (0.45ng/ml) were placed and cultured for 24 hours; (3) The morphological changes of RLE exposed to different concentrations of Hh, 5-Fu and ADM were studied under light microscope.. The ID50 of Hh, 5-Fu and ADM exposed to RLE for 24 hours were 0.84 microgram/ml, 0.58 microgram/ml and 4.50ng/ml, respectively, and those for 72 hours were 0.49 microgram/ml, 0.33 microgram/ml and 3.85ng/ml. The attachment rate of RLE after being cultured for 24 hours with Hh, 5-Fu and ADM were respectively 83.6%, 89.1% and 87.3%. The morphological changes of RLE demonstrated that obvious changes in the cell membrane and cytoplasm were found even in lower concentration, but changes in the nuclei could only be found in higher concentration of these drugs.. Hh can not only inhibit the proliferation of RLE but also reduce the number of attached cells. It is suggested that Hh may be more useful for the prevention of after cataract than 5-Fu and ADM.

Topics: Animals; Cataract; Cell Adhesion; Cell Division; Cells, Cultured; Epithelial Cells; Female; Growth Inhibitors; Harringtonines; Homoharringtonine; In Vitro Techniques; Lens, Crystalline; Male; Rabbits

To study the effects of Homoharringtonine (Hh) on lens epithelial cells (LEC) in rabbits for the possibility of preventing or treating the development of after cataract.. Lens epithelial cells from the right eyes of 9 New Zealand Albino rabbits were processed for light microscope and transmission electronic microscope 30 minutes, 3 days and 60 days after phacoemulsification by the use of Hh (20 micrograms/ ml) in balanced salt solution (BSS) as irrigating solution. LEC from the left eyes of the same rabbits served as controls after phacoemulsification by the use of BSS. Clinical examinations were also performed in all eyes before and after the surgery.. Hh as irrigating solution during phacoemulsification had antiproliferative effects on LEC 3 days postoperatively, the Hh treated eyes developed less LEC proliferation than the controls. Though the development of after cataract formation (Soemmering ring) could not be prevented in both Hh treated eyes and controls 60 days after phacoemulsification, the ultrastructural study of LEC from the Hh treated eyes indicated some changes of cytoplasm such as vacuolation of organelles.. It is suggested that increasing the concentration of Hh in irrigating solution for phacoemulsification and prolonging the existence of Hh in aqueous humor may have more effects on the inhibition of the proliferation of LEC. From the results of this preliminary study a pharmacological means of prevention and treatment of after cataract may be indicated.

Topics: Animals; Cataract; Cell Division; Epithelium; Harringtonines; Homoharringtonine; Lens, Crystalline; Male; Phacoemulsification; Rabbits; Random Allocation