hispidulin and Leukemia

To investigate the effect and underlying mechanism of hispidulin on the proliferation and apoptosis of leukemia K562 cells.. K562 cells were cultured in vitro and treated with 0, 5, 25 or 100 μmol/L hispidulin for 24 h. Cell proliferation and apoptosis were detected by CCK-8 and flow cytometry, respectively. Western blot was used to assess the expression of Bax, Bcl-2 and interleukin (IL)-37 proteins. Bone marrow mononuclear cells were extracted from 17 chronic myeloid leukemia patients and 21 healthy individuals by Ficoll-Hypaque density gradient method, and the expression of IL-37 protein was measured by Western blot. K562 cells with IL-37 overexpression or knockdown were constructed, and then treated with 0 or 100 μmol/L hispidulin for 24 h. Cell proliferation, apoptosis and protein expression of Bax and Bcl-2 were determined in the same way as above.. After K562 cells were treated with hispidulin, the cell inhibition rate, apoptosis rate, and the protein expression of Bax and IL-37 were significantly increased (. Hispidulin inhibits the proliferation and induces apoptosis of leukemia K562 cells, which may be related to the up-regulation of IL-37 protein in cells.. 高车前素通过上调IL-37影响白血病K562细胞增殖和凋亡的实验研究.. 探讨高车前素对白血病K562细胞增殖和凋亡的影响及可能机制。.. 体外培养K562细胞，分别用高车前素0、5、25、100 μmol/L作用24 h，CCK-8法检测细胞增殖，流式细胞术检测细胞凋亡，蛋白质印迹法检测Bax、Bcl-2和白细胞介素（IL）-37蛋白表达。利用Ficoll-Hypaque密度梯度法提取17例慢性髓系白血病患者和21例健康体检者骨髓单个核细胞，蛋白质印迹法检测IL-37蛋白表达。构建过表达或敲减IL-37的K562细胞，再用高车前素0、100 μmol/L作用24 h，上述相同方法观察细胞增殖、凋亡及Bax和Bcl-2蛋白表达。.. K562细胞经高车前素干预后，细胞增殖抑制率、凋亡率及Bax和IL-37蛋白表达均显著升高（. 高车前素抑制白血病K562细胞增殖，并诱导细胞凋亡，这可能与其上调细胞中IL-37蛋白表达有关。.

Topics: Apoptosis; bcl-2-Associated X Protein; Cell Proliferation; Humans; K562 Cells; Leukemia; Proto-Oncogene Proteins c-bcl-2