hes1-protein--human and Biliary-Atresia

To analyze the expression and function of the Notch signaling target gene Hes1 in a rhesus rotavirus-induced mouse biliary atresia model.. The morphologies of biliary epithelial cells in biliary atresia patients and in a mouse model were examined by immunohistochemical staining. Then, the differential expression of Notch signaling pathway-related molecules was investigated. Further, the effects of the siRNA-mediated inhibition of Hes1 expression were examined using a biliary epithelial cell 3D culture system.. Both immature (EpCAM. Our data indicated that Hes1 might contribute to the maturation and the cellular structure organization of biliary epithelial cells, which provides new insight into understanding the pathology of biliary atresia.

Topics: Animals; Bile Ducts; Biliary Atresia; Cell Culture Techniques; Cells, Cultured; Choledochal Cyst; Disease Models, Animal; Down-Regulation; Epithelial Cells; Female; Gene Expression Profiling; Humans; Immunoglobulin J Recombination Signal Sequence-Binding Protein; Liver; Mice; Mice, Inbred BALB C; Microscopy, Electron; Receptors, Notch; RNA Interference; RNA, Small Interfering; Rotavirus; Signal Transduction; Transcription Factor HES-1