herbimycin and Hodgkin-Disease

herbimycin has been researched along with Hodgkin-Disease* in 1 studies

Other Studies

1 other study(ies) available for herbimycin and Hodgkin-Disease

Article	Year
Inhibition of tyrosine kinase activity induces caspase-dependent apoptosis in anaplastic large cell lymphoma with NPM-ALK (p80) fusion protein. Experimental hematology, 2001, Volume: 29, Issue:9 The t(2;5)(p23;q35) translocation creates a fusion gene NPM-ALK (p80) that encodes a product with tyrosine kinase activity believed to play an important role in development of anaplastic large cell lymphoma (ALCL). Our study was aimed to analyze tyrosine kinase activity and phosphotyrosine in ALCLs. We were also interested in determining the effect of tyrosine kinase inhibitors on survival of ALCL.. Eleven cases of ALCL and three ALCL cell lines with t(2;5)(Karpas-299, SUPM2, SU-DHL-1) and 10 Hodgkin's disease (HD) samples were stained with anti-phosphotyrosine antibody. The tyrosine kinase activity, p80 phosphorylation, and the apoptotic effects of two tyrosine kinase inhibitors, herbimycin A and STI-571, were determined on ALCL cell lines.. Herbimycin A had showed both a time- and dose-dependent apoptotic effect on all three cell lines, while STI-571 demonstrated a minimal effect. Following herbimycin A treatment, a decrease in tyrosine kinase activity in the ALCL cell lines and inhibition in NPM-ALK (p80) autophosphorylation was demonstrated by immunoprecipitation and Western blotting. Herbimycin A-induced apoptosis was accompanied by caspase-3 activation. Furthermore, apoptosis induced by herbimycin A was blocked by both z-VAD-FMK and z-DEVD-FMK, suggesting a critical role of caspases.. These findings indicate that tyrosine kinase activity is a common characteristic of ALCLs and necessary for ALCL cell survival. These findings further suggest that therapies targeting tyrosine kinases, including p80, may have clinical utility. Topics: Anaplastic Lymphoma Kinase; Antineoplastic Agents; Apoptosis; Benzamides; Benzoquinones; Caspases; Chromosomes, Human, Pair 2; Chromosomes, Human, Pair 5; Enzyme Inhibitors; Hodgkin Disease; Humans; Imatinib Mesylate; Immunohistochemistry; Lactams, Macrocyclic; Lymphoma, Large B-Cell, Diffuse; Oncogene Proteins, Fusion; Phosphorylation; Piperazines; Protein-Tyrosine Kinases; Pyrimidines; Quinones; Receptor Protein-Tyrosine Kinases; Rifabutin; Translocation, Genetic; Tumor Cells, Cultured	2001

Article

Year

Inhibition of tyrosine kinase activity induces caspase-dependent apoptosis in anaplastic large cell lymphoma with NPM-ALK (p80) fusion protein.

Experimental hematology, 2001, Volume: 29, Issue:9

The t(2;5)(p23;q35) translocation creates a fusion gene NPM-ALK (p80) that encodes a product with tyrosine kinase activity believed to play an important role in development of anaplastic large cell lymphoma (ALCL). Our study was aimed to analyze tyrosine kinase activity and phosphotyrosine in ALCLs. We were also interested in determining the effect of tyrosine kinase inhibitors on survival of ALCL.. Eleven cases of ALCL and three ALCL cell lines with t(2;5)(Karpas-299, SUPM2, SU-DHL-1) and 10 Hodgkin's disease (HD) samples were stained with anti-phosphotyrosine antibody. The tyrosine kinase activity, p80 phosphorylation, and the apoptotic effects of two tyrosine kinase inhibitors, herbimycin A and STI-571, were determined on ALCL cell lines.. Herbimycin A had showed both a time- and dose-dependent apoptotic effect on all three cell lines, while STI-571 demonstrated a minimal effect. Following herbimycin A treatment, a decrease in tyrosine kinase activity in the ALCL cell lines and inhibition in NPM-ALK (p80) autophosphorylation was demonstrated by immunoprecipitation and Western blotting. Herbimycin A-induced apoptosis was accompanied by caspase-3 activation. Furthermore, apoptosis induced by herbimycin A was blocked by both z-VAD-FMK and z-DEVD-FMK, suggesting a critical role of caspases.. These findings indicate that tyrosine kinase activity is a common characteristic of ALCLs and necessary for ALCL cell survival. These findings further suggest that therapies targeting tyrosine kinases, including p80, may have clinical utility.

Topics: Anaplastic Lymphoma Kinase; Antineoplastic Agents; Apoptosis; Benzamides; Benzoquinones; Caspases; Chromosomes, Human, Pair 2; Chromosomes, Human, Pair 5; Enzyme Inhibitors; Hodgkin Disease; Humans; Imatinib Mesylate; Immunohistochemistry; Lactams, Macrocyclic; Lymphoma, Large B-Cell, Diffuse; Oncogene Proteins, Fusion; Phosphorylation; Piperazines; Protein-Tyrosine Kinases; Pyrimidines; Quinones; Receptor Protein-Tyrosine Kinases; Rifabutin; Translocation, Genetic; Tumor Cells, Cultured

2001