heparitin-sulfate and Periodontal-Diseases

The glycosylaminoglycan (GAG) distribution in the soft periodontal tissues of the sheep was investigated topographically in healthy tissue and the changes associated with periodontal disease determined and correlated with increasing disease severity. Total sulphated GAG content was determined spectrophotometrically and the proportions of individual GAGS measured after separation by cellulose acetate electrophoresis. In healthy tissue, total sulphated GAG distribution was found to mirror that described previously for collagen, being highest in the gum-pad region and adjacent to alveolar bone. This changed markedly in relation to periodontal disease. Early disease-related changes in GAGs were first detected adjacent to alveolar bone. In severely diseases tissue, an overall increase in sulphated GAG, particularly in the gum-pad region, was present. Increasing disease severity was accompanied by a decrease in dermatan sulphate and a concomitant increase in the proportion of chondroitin-4-sulphate. These changes may be due to the combined effects of chronic inflammation and alterations to the mechanical loading of the tissue owing to loss of attachment.

Topics: Animals; Chondroitin Sulfates; Collagen; Densitometry; Dermatan Sulfate; Electrophoresis, Cellulose Acetate; Female; Glycosaminoglycans; Heparitin Sulfate; Hyaluronic Acid; Lasers; Periodontal Diseases; Periodontium; Sheep

Topics: Chondroitin Sulfates; Electrophoresis, Cellulose Acetate; Gingival Crevicular Fluid; Gingivitis; Glycosaminoglycans; Heparitin Sulfate; Humans; Hyaluronic Acid; Periodontal Diseases