heparitin-sulfate and Gingivitis

Glycosaminoglycans were extracted from normal, inflamed and phenytoin induced overgrowth of human gingival tissue by proteolysis and alcohol precipitation. Extracts were run in a Dowex-1 column and the fractions were treated with mucopolysaccharidases. Cellulose acetate electrophoresis was carried out with or without enzyme digestion for identification of individual glycosaminoglycans. Glycosaminoglycans were found to be decreased in inflammation but were observed to increase in the overgrowth. Hyaluronic acid was found to be increased in both the pathological conditions. Dermatan sulphate, chondroitin sulphate and heparan sulphate were observed to be decreased in inflammation. In overgrowth, dermatan sulphate and chondroitin sulphate were found to increase while the presence of heparan sulphate was not significant. The changes in the pattern of individual glycosaminoglycan in the two varied conditions are discussed.

Topics: Chondroitin Sulfates; Dermatan Sulfate; Gingiva; Gingivitis; Heparitin Sulfate; Humans; Hyaluronic Acid; Phenytoin

Proteoglycans were extracted from human gingiva with 2 M CaCl2. The extracts were examined by gel filtration on Sephacryl S-400 in 2 M CaCl2 under dissociative conditions. The 280 nm absorbance profiles of clinically uninflamed, inflamed and severely-inflamed tissues showed that material was present with molecular weights of between 2 X 10(6) or greater, and 16,000. Proteoglycans were examined by cellulose-acetate electrophoresis with subsequent identification of the constituent glycosaminoglycans after protease digestion, and finally by chondroitinase AC digestion of the liberated glycosaminoglycans. The relative proportion of each glycosaminoglycan was calculated by scanning each cellulose-acetate sheet on an integrating densitometer. Heparan sulphate was found only in fraction I (mol. wt 2 X 10(6) or greater), together with hyaluronic acid and chondroitin-4-sulphate, these being present in all of the glycosaminoglycan-containing fractions (I-IV). Dermatan sulphate was absent from fraction I, but present in II-IV, apparently existing on the same protein core as chondroitin-4-sulphate. The relative proportions of these two glycosaminoglycans was related to molecular size, and with the degree of inflammation for a given molecular species.

Topics: Chondroitin Sulfates; Chromatography, Gel; Dermatan Sulfate; Electrophoresis, Cellulose Acetate; Gingiva; Gingivitis; Glycosaminoglycans; Heparitin Sulfate; Humans; Hyaluronic Acid; Proteoglycans

Topics: Chondroitin Sulfates; Electrophoresis, Cellulose Acetate; Gingival Crevicular Fluid; Gingivitis; Glycosaminoglycans; Heparitin Sulfate; Humans; Hyaluronic Acid; Periodontal Diseases

Topics: Alveolar Process; Animals; Bone Resorption; Chondroitin Sulfates; Dermatan Sulfate; Dogs; Gingivitis; Glycosaminoglycans; Heparitin Sulfate; Hyaluronic Acid; Periodontitis