heparitin-sulfate and Carcinoma--Ehrlich-Tumor

heparitin-sulfate has been researched along with Carcinoma--Ehrlich-Tumor* in 2 studies

Other Studies

2 other study(ies) available for heparitin-sulfate and Carcinoma--Ehrlich-Tumor

Article	Year
67Ga uptake and heparan sulfate content of Ehrlich solid tumor in mice. European journal of nuclear medicine, 1986, Volume: 12, Issue:4 The relationship between 67Ga uptake and heparan sulfate (HS) content in Ehrlich solid tumor (EST) of mice was investigated, and the effect of cyanomethylamine, papain, streptozotocin, or bleomycin pretreatment on 67Ga uptake in EST was studied. 67Ga uptakes in EST and kidney were much higher than other tissues, and these tissues also contained large amounts of HS. 67Ga uptakes and HS synthesis in the EST were inhibited by pretreatment with cyanomethylamine or papain (inhibitors of fibrosis). Parallel reductions of 67Ga uptake and HS synthesis in EST were observed in EST transplanted into streptozotocin-induced diabetic mice. The weight of EST in the bleomycin-injected group was decreased to less than half of the control, but no effect was observed on 67Ga uptake per gram of EST. These results suggest that 67Ga uptake in the tumor and inflammatory lesions are related to the quantity of HS in these tissues, and the correlation between the uptake of 67Ga and the rate of cellular proliferation is secondary. Topics: Aminoacetonitrile; Animals; Bleomycin; Carcinoma, Ehrlich Tumor; Electrophoresis, Cellulose Acetate; Gallium Radioisotopes; Glycosaminoglycans; Heparitin Sulfate; Hyaluronic Acid; Kidney; Male; Mice; Mice, Inbred Strains; Muscles; Papain; Streptozocin	1986
In vitro binding of 67Ga to Ehrlich ascites tumor cells. European journal of nuclear medicine, 1984, Volume: 9, Issue:8 The binding of 67Ga to Ehrlich ascites tumor cells (ETC) was studied in vitro. Acid mucopolysaccharide (AMPS) present at the cell surface of ETC was identified as heparan sulfate (HS). The extent of 67Ga binding to ETC reached a plateau (ca. 10% of the added dose) at 1-2 h after the start of incubation. The binding was higher under neutral or alkaline conditions than under acidic conditions. Heparin and heparitinase treatment both significantly decreased the extent of 67Ga binding to ETC. Mild treatment with protease, including trypsin or papain, also decreased the binding. On the contrary, the treatment with trypsin under severe conditions markedly increased the extent of 67Ga binding to ETC. These results support the hypothesis that HS plays an important role as a 67Ga receptor in the mechanism of gallium binding to ETC. Topics: Animals; Binding Sites; Carcinoma, Ehrlich Tumor; Gallium Radioisotopes; Heparin; Heparitin Sulfate; Mice; Peptide Hydrolases; Polysaccharide-Lyases	1984

Article

Year

67Ga uptake and heparan sulfate content of Ehrlich solid tumor in mice.

European journal of nuclear medicine, 1986, Volume: 12, Issue:4

The relationship between 67Ga uptake and heparan sulfate (HS) content in Ehrlich solid tumor (EST) of mice was investigated, and the effect of cyanomethylamine, papain, streptozotocin, or bleomycin pretreatment on 67Ga uptake in EST was studied. 67Ga uptakes in EST and kidney were much higher than other tissues, and these tissues also contained large amounts of HS. 67Ga uptakes and HS synthesis in the EST were inhibited by pretreatment with cyanomethylamine or papain (inhibitors of fibrosis). Parallel reductions of 67Ga uptake and HS synthesis in EST were observed in EST transplanted into streptozotocin-induced diabetic mice. The weight of EST in the bleomycin-injected group was decreased to less than half of the control, but no effect was observed on 67Ga uptake per gram of EST. These results suggest that 67Ga uptake in the tumor and inflammatory lesions are related to the quantity of HS in these tissues, and the correlation between the uptake of 67Ga and the rate of cellular proliferation is secondary.

Topics: Aminoacetonitrile; Animals; Bleomycin; Carcinoma, Ehrlich Tumor; Electrophoresis, Cellulose Acetate; Gallium Radioisotopes; Glycosaminoglycans; Heparitin Sulfate; Hyaluronic Acid; Kidney; Male; Mice; Mice, Inbred Strains; Muscles; Papain; Streptozocin

1986

In vitro binding of 67Ga to Ehrlich ascites tumor cells.

European journal of nuclear medicine, 1984, Volume: 9, Issue:8

The binding of 67Ga to Ehrlich ascites tumor cells (ETC) was studied in vitro. Acid mucopolysaccharide (AMPS) present at the cell surface of ETC was identified as heparan sulfate (HS). The extent of 67Ga binding to ETC reached a plateau (ca. 10% of the added dose) at 1-2 h after the start of incubation. The binding was higher under neutral or alkaline conditions than under acidic conditions. Heparin and heparitinase treatment both significantly decreased the extent of 67Ga binding to ETC. Mild treatment with protease, including trypsin or papain, also decreased the binding. On the contrary, the treatment with trypsin under severe conditions markedly increased the extent of 67Ga binding to ETC. These results support the hypothesis that HS plays an important role as a 67Ga receptor in the mechanism of gallium binding to ETC.

Topics: Animals; Binding Sites; Carcinoma, Ehrlich Tumor; Gallium Radioisotopes; Heparin; Heparitin Sulfate; Mice; Peptide Hydrolases; Polysaccharide-Lyases

1984