heparitin-sulfate and Carcinoma--Adenoid-Cystic

In order to reconstruct the characteristic three-dimensional architecture of adenoid cystic carcinoma, we cultured ACC2 cells, a cell system established from a human adenoid cystic carcinoma of the palate, in collagen gel matrix and transplanted them in SCID mice. In the collagen gel culture, the cells formed spherical colonies measuring 75.6 +/- 14.6 microns in diameter by 6 days after seeding. The tumor cell nests contained vacuolar structures that were immunopositive for heparan sulfate proteoglycan, type III collagen, type IV collagen, and fibronectin. The rim of the nests was argyrophilic and immunopositive for type I collagen, type IV collagen, laminin, and fibronectin. Transplants of ACC2 cells in SCID mice grew to form tumor masses in which pseudocysts were formed. The results indicate that our collagen gel culture system provides physiological conditions for ACC2 cells to secrete particular extracellular matrix molecules and form pseudocystic spaces.

Topics: Animals; Carcinoma, Adenoid Cystic; Collagen; Coloring Agents; Culture Media; Cysts; Extracellular Matrix Proteins; Fibronectins; Gels; Heparitin Sulfate; Humans; Laminin; Mice; Mice, SCID; Neoplasm Transplantation; Palatal Neoplasms; Silver; Transplantation, Heterologous; Tumor Cells, Cultured; Vacuoles

Tissue samples from 30 patients with adenoid cystic carcinoma and 20 with adenocarcinoma of salivary gland origin were studied by immunohistochemical staining with specific antibodies to the four macromolecules that are present in normal basement membranes: type IV collagen, laminin, heparan sulfate proteoglycan, and entactin. In the adenoid cystic carcinoma samples, the four proteins were localized in different types of extracellular matrices in the tumor, namely pseudocystic spaces, hyaline stroma, and around tumor cell nests. The staining intensity was enhanced by pretreatment with hyaluronidase. The tumor cells of adenoid cystic carcinoma showed a tendency to proliferate with individual cells in contact with the basement membrane and to infiltrate through basement membrane-rich tissues, such as peripheral nerves, blood vessels, and skeletal muscles. In contrast, only circumferential staining of tumor cell nests was obtained in adenocarcinoma samples. The results suggest that adenoid cystic carcinoma is a tumor with affinity for basement membranes, and this basic feature is reflected in its histology and presumably in its biologic behavior. Immunostaining with antibodies to basement membrane proteins appears to be useful for differential diagnosis of some types of these two carcinomas.

Topics: Adenocarcinoma; Basement Membrane; Carcinoma, Adenoid Cystic; Collagen; Heparan Sulfate Proteoglycans; Heparitin Sulfate; Humans; Immunohistochemistry; Laminin; Membrane Glycoproteins; Neoplasm Proteins; Proteoglycans

A new cell line has been established from an adenoid cystic carcinoma arising in the submandibular gland of a 63-year-old woman. The cultured epithelial-like cells grew vigorously and adhered together to form a sheet. Immunohistochemical stainings for type IV collagen, laminin and fibronectin were clearly positive in the intercellular matrix and on the surface of the culture cells. Chondroitin 6-sulfate proteoglycan and heparan sulfate were also detected. Ultrastructural studies showed that the cells had abundant rough endoplasmic reticulum and a well-developed Golgi apparatus. Rough endoplasmic reticulum near the cell surface was markedly dilated, and contained material of low electron density. This cell line would be useful for biological and biochemical studies on the mechanisms by which the stromal component is formed.

Topics: Basement Membrane; Carcinoma, Adenoid Cystic; Collagen; Female; Heparitin Sulfate; Humans; Immunohistochemistry; Laminin; Microscopy, Electron; Microscopy, Phase-Contrast; Middle Aged; Proteoglycans; Salivary Gland Neoplasms; Tumor Cells, Cultured

Specific antibodies against basement membrane associated, connective tissue components: type IV and V collagens, laminin, fibronectin and heparan sulphate proteoglycan were used to study the basement membrane-like structures in cylindroma lesions. All these components were immunohistochemically demonstrated as a band surrounding islands of epithelial cells and all except fibronectin also inside the islands. Antibodies to keratin filaments stained most of the cells inside the epithelial islands confirming the epithelial origin of the cells.

Topics: Basement Membrane; Carcinoma, Adenoid Cystic; Chondroitin Sulfate Proteoglycans; Collagen; Female; Fibronectins; Fluorescent Antibody Technique; Genes, Dominant; Heparan Sulfate Proteoglycans; Heparitin Sulfate; Humans; Keratins; Laminin; Middle Aged; Skin Neoplasms