heparitin-sulfate and Bunyaviridae-Infections

Schmallenberg virus (SBV) is an insect-transmitted orthobunyavirus that can cause abortions and congenital malformations in the offspring of ruminants. Even though the two viral surface glycoproteins Gn and Gc are involved in host cell entry, the specific cellular receptors of SBV are currently unknown. Using genome-wide CRISPR-Cas9 forward screening, we identified 3'-phosphoadenosine 5'-phosphosulfate (PAPS) transporter 1 (PAPST1) as an essential factor for SBV infection. PAPST1 is a sulfotransferase involved in heparan sulfate proteoglycan synthesis encoded by the solute carrier family 35 member B2 gene (

Topics: Animals; Bunyaviridae; Bunyaviridae Infections; Chlorocebus aethiops; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR-Cas Systems; Europe; Gene Knockout Techniques; HEK293 Cells; Heparitin Sulfate; Humans; Livestock; Membrane Glycoproteins; Orthobunyavirus; Rift Valley fever virus; Sulfate Transporters; Sulfotransferases; Vero Cells; Virus Attachment