h-85 and Leukemia-P388

h-85 has been researched along with Leukemia-P388* in 1 studies

Other Studies

1 other study(ies) available for h-85 and Leukemia-P388

Article	Year
Obviation of drug resistance and affinity purification of P-glycoprotein by isoquinolinesulfonamides. Cancer letters, 1991, Volume: 60, Issue:2 A newly synthesized isoquinolinesulfonamide named H-85; N-[2-[N-formyl-N-[[3-(4-chlorophenyl)-2-propenyl] amino] ethyl]-5-isoquinolinesulfonamide was found to reverse drug resistance in multidrug resistant P388 murine leukemic cells (P388/ADR). The energy-dependent extrusion of [3H]vinblastine from P388/ADR-cells was significantly suppressed by 10 microM H-85 but not so the efflux from the sensitive P388 cells. A 140-kDa protein overexpressed in P388/ADR cells was photoaffinity labeled with a vinblastine analog; N-(P-azid-[3-125I]salicyl-N'-(beta-aminoethyl) vindesine and H-85 selectively inhibited photolabeling of the 140-kDa protein. This 140-kDa protein was purified to apparent homogeneity by succeeding steps of phosphocellulose, DEAE-cellulose, and W-66 (a derivative of H-85)-coupled sepharose chromatography. The purified 140-kDa protein proved to be immunopositive with the P-glycoprotein-specific monoclonal antibody, C219. Topics: Animals; ATP Binding Cassette Transporter, Subfamily B, Member 1; Chromatography, Affinity; Chromatography, DEAE-Cellulose; Dose-Response Relationship, Drug; Drug Resistance; Electrophoresis, Polyacrylamide Gel; Female; In Vitro Techniques; Isoquinolines; Leukemia P388; Membrane Glycoproteins; Mice; Tumor Cells, Cultured; Vinblastine	1991

Article

Year

Obviation of drug resistance and affinity purification of P-glycoprotein by isoquinolinesulfonamides.

Cancer letters, 1991, Volume: 60, Issue:2

A newly synthesized isoquinolinesulfonamide named H-85; N-[2-[N-formyl-N-[[3-(4-chlorophenyl)-2-propenyl] amino] ethyl]-5-isoquinolinesulfonamide was found to reverse drug resistance in multidrug resistant P388 murine leukemic cells (P388/ADR). The energy-dependent extrusion of [3H]vinblastine from P388/ADR-cells was significantly suppressed by 10 microM H-85 but not so the efflux from the sensitive P388 cells. A 140-kDa protein overexpressed in P388/ADR cells was photoaffinity labeled with a vinblastine analog; N-(P-azid-[3-125I]salicyl-N'-(beta-aminoethyl) vindesine and H-85 selectively inhibited photolabeling of the 140-kDa protein. This 140-kDa protein was purified to apparent homogeneity by succeeding steps of phosphocellulose, DEAE-cellulose, and W-66 (a derivative of H-85)-coupled sepharose chromatography. The purified 140-kDa protein proved to be immunopositive with the P-glycoprotein-specific monoclonal antibody, C219.

Topics: Animals; ATP Binding Cassette Transporter, Subfamily B, Member 1; Chromatography, Affinity; Chromatography, DEAE-Cellulose; Dose-Response Relationship, Drug; Drug Resistance; Electrophoresis, Polyacrylamide Gel; Female; In Vitro Techniques; Isoquinolines; Leukemia P388; Membrane Glycoproteins; Mice; Tumor Cells, Cultured; Vinblastine

1991