guanylyl-imidodiphosphate and Stomach-Neoplasms

guanylyl-imidodiphosphate has been researched along with Stomach-Neoplasms* in 2 studies

Other Studies

2 other study(ies) available for guanylyl-imidodiphosphate and Stomach-Neoplasms

Article	Year
A somatostatin receptor negatively coupled to adenylate cyclase in the human gastric cell line HGT-1. Regulatory peptides, 1986, Dec-30, Volume: 16, Issue:3-4 Somatostatin receptors are demonstrated in the human derived gastric cell line HGT-1. Using 125I-Tyr11-somatostatin as ligand, two classes of sites were characterized with apparent dissociation constants KD1 = 0.9 X 10(-10) M and KD2 = 4 X 10(-9) M and maximum binding capacities of N1 = 20 and N2 = 556 fmol per mg protein, respectively. These values are close to those previously reported in freshly isolated parietal cells (Reyl, F., Silve, C. and Lewin, M.J.M., Somatostatin receptors on isolated gastric cells. In S. Bonfils et al. (Eds.), Hormone Receptors in Digestion and Nutrition, Elsevier/North-Holland, Amsterdam, 1979, pp. 391-400). Somatostatin binding to the high affinity sites was partially inhibited by the non-hydrolysable guanyl nucleotide analog Gpp(NH)p and by pretreating the cells with islet activating protein (IAP). Furthermore, IAP counteracted the inhibitory effect of somatostatin on histamine stimulation of adenylate cyclase. These findings are interpreted in terms of somatostatin interaction with the 41,000 Da adenylate cyclase GTP-dependent inhibitory subunit, Ni. Topics: Adenylate Cyclase Toxin; Adenylyl Cyclases; Cell Line; Guanylyl Imidodiphosphate; Histamine; Humans; Kinetics; Pertussis Toxin; Receptors, Neurotransmitter; Receptors, Somatostatin; Somatostatin; Stomach; Stomach Neoplasms; Virulence Factors, Bordetella	1986
Highly histamine-responsive clones from the human gastric adenocarcinoma cell line HGT-1. Agents and actions, 1986, Volume: 17, Issue:5-6 The human gastric epithelial cell line HGT-1 possesses adenylate cyclase-coupled histamine H2 receptors. To test the cellular homogeneity or heterogeneity with respect to these receptors, we have isolated 7 clones from the HGT-1 line and studied their basal and histamine-stimulated adenylate cyclase activities. Basal adenylate cyclase activities of the clones did not differ significantly, nor did 10 mM NaF- nor 0.1 mM Gpp(NH)p-stimulated activities. However, histamine stimulation of adenylate cyclase varied among clones from 1.9 fold to 5.4 fold basal activity. The EC50 values, determined in 3 clones, were not significantly different. These findings support the heterogeneity of histamine responsiveness of the human gastric cell line HGT-1. In addition, they suggest that highly histamine-responsive clones may be useful models to study the gastric histamine H2-receptor and its specific antagonists in the human. Topics: Adenocarcinoma; Adenylyl Cyclases; Cell Line; Cimetidine; Clone Cells; Diphenhydramine; Guanylyl Imidodiphosphate; Histamine; Humans; Kinetics; Sodium Fluoride; Stomach Neoplasms	1986

Article

Year

A somatostatin receptor negatively coupled to adenylate cyclase in the human gastric cell line HGT-1.

Regulatory peptides, 1986, Dec-30, Volume: 16, Issue:3-4

Somatostatin receptors are demonstrated in the human derived gastric cell line HGT-1. Using 125I-Tyr11-somatostatin as ligand, two classes of sites were characterized with apparent dissociation constants KD1 = 0.9 X 10(-10) M and KD2 = 4 X 10(-9) M and maximum binding capacities of N1 = 20 and N2 = 556 fmol per mg protein, respectively. These values are close to those previously reported in freshly isolated parietal cells (Reyl, F., Silve, C. and Lewin, M.J.M., Somatostatin receptors on isolated gastric cells. In S. Bonfils et al. (Eds.), Hormone Receptors in Digestion and Nutrition, Elsevier/North-Holland, Amsterdam, 1979, pp. 391-400). Somatostatin binding to the high affinity sites was partially inhibited by the non-hydrolysable guanyl nucleotide analog Gpp(NH)p and by pretreating the cells with islet activating protein (IAP). Furthermore, IAP counteracted the inhibitory effect of somatostatin on histamine stimulation of adenylate cyclase. These findings are interpreted in terms of somatostatin interaction with the 41,000 Da adenylate cyclase GTP-dependent inhibitory subunit, Ni.

Topics: Adenylate Cyclase Toxin; Adenylyl Cyclases; Cell Line; Guanylyl Imidodiphosphate; Histamine; Humans; Kinetics; Pertussis Toxin; Receptors, Neurotransmitter; Receptors, Somatostatin; Somatostatin; Stomach; Stomach Neoplasms; Virulence Factors, Bordetella

1986

Highly histamine-responsive clones from the human gastric adenocarcinoma cell line HGT-1.

Agents and actions, 1986, Volume: 17, Issue:5-6

The human gastric epithelial cell line HGT-1 possesses adenylate cyclase-coupled histamine H2 receptors. To test the cellular homogeneity or heterogeneity with respect to these receptors, we have isolated 7 clones from the HGT-1 line and studied their basal and histamine-stimulated adenylate cyclase activities. Basal adenylate cyclase activities of the clones did not differ significantly, nor did 10 mM NaF- nor 0.1 mM Gpp(NH)p-stimulated activities. However, histamine stimulation of adenylate cyclase varied among clones from 1.9 fold to 5.4 fold basal activity. The EC50 values, determined in 3 clones, were not significantly different. These findings support the heterogeneity of histamine responsiveness of the human gastric cell line HGT-1. In addition, they suggest that highly histamine-responsive clones may be useful models to study the gastric histamine H2-receptor and its specific antagonists in the human.

Topics: Adenocarcinoma; Adenylyl Cyclases; Cell Line; Cimetidine; Clone Cells; Diphenhydramine; Guanylyl Imidodiphosphate; Histamine; Humans; Kinetics; Sodium Fluoride; Stomach Neoplasms

1986