guanylyl-(3--5-)-guanosine has been researched along with Carcinoma--Non-Small-Cell-Lung* in 1 studies
1 other study(ies) available for guanylyl-(3--5-)-guanosine and Carcinoma--Non-Small-Cell-Lung
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Formation and repair kinetics of Pt-(GpG) DNA adducts in extracted circulating tumour cells and response to platinum treatment.
Pt-(GpG) intrastrand crosslinks are the major DNA adducts induced by platinum-based anticancer drugs. In the cell lines and mouse models, the persistence of these lesions correlates significantly with cell damage. Here we studied Pt-(GpG) DNA adducts in circulating tumour cells (CTC) treated with cisplatin in medium upfront to systemic therapy from patients with advanced non-small-cell lung cancer (NSCLC).. Blood was drawn before systemic treatment and the CD45/CD15-depleted fraction of mononuclear cells was exposed to cisplatin, verified for the presence of CTC by pan-cytokeratin (pCK) staining and immunoanalysed for the level of Pt-(GpG) in DNA.. Immunostaining for pCK, CD45 and subsequently for Pt-(GpG) adducts in the cisplatin-exposed cells (ex vivo) at different time points depicted distinct differences for adduct persistence in CTC between responders vs non-responders.. Pt-(GpG) adducts can be detected in CTC from NSCLC patients and assessing their kinetics may constitute a clinically feasible biomarker for response prediction and dose individualisation of platinum-based chemotherapy. This functional pre-therapeutic test might represent a more biological approach than measuring protein factors or other molecular markers. Topics: Aged; Carcinoma, Non-Small-Cell Lung; Cisplatin; Dinucleoside Phosphates; DNA Adducts; Humans; Leukocyte Common Antigens; Leukocytes, Mononuclear; Lewis X Antigen; Lung Neoplasms; Middle Aged; Neoplastic Cells, Circulating; Platinum; Tumor Cells, Cultured | 2013 |