guanylin and Adenoma

guanylin has been researched along with Adenoma* in 2 studies

Other Studies

2 other study(ies) available for guanylin and Adenoma

ArticleYear
Guanylin and functional coupling proteins in the human salivary glands and gland tumors : expression, cellular localization, and target membrane domains.
    The American journal of pathology, 2002, Volume: 161, Issue:2

    Cystic fibrosis transmembrane conductance regulator (CFTR)-mediated secretion of an electrolyte-rich fluid is a major but incompletely understood function of the salivary glands. We provide molecular evidence that guanylin, a bioactive intestinal peptide involved in the CFTR-regulated secretion of electrolyte/water in the gut epithelium, is highly expressed in the human parotid and submandibular glands and in respective clinically most relevant tumors. Moreover, in the same organs we identified expression of the major components of the guanylin signaling pathway, ie, guanylin-receptor guanylate cyclase-C, cGKII, and CFTR, as well as of the epithelial Cl(-)/HCO(3)(-) anion exchanger type 2 (AE2). At the cellular level, guanylin is localized to epithelial cells of the ductal system that, based on its presence in the saliva, is obviously released into the salivary gland ducts. The guanylin-receptor guanylate cyclase-C, cGKII, CFTR, and AE2 are all confined exclusively to the apical membrane of the same duct cells. These findings implicate guanylin as intrinsic regulator of electrolyte secretion in the salivary glands. We assume that duct epithelial cells synthesize and release guanylin into the saliva to regulate electrolyte secretion in the ductal system by an intraductal luminocrine signaling pathway. Moreover, the high expression of guanylin in pleomorphic adenoma and Warthin tumors (cystadenolymphoma), the most common neoplasms of salivary glands, predicts guanylin as a significant marker in tumor pathology.

    Topics: Adenolymphoma; Adenoma; Biomarkers, Tumor; Cystic Fibrosis Transmembrane Conductance Regulator; Gastrointestinal Hormones; Humans; Natriuretic Peptides; Parotid Gland; Peptides; Protein Structure, Tertiary; Salivary Gland Neoplasms; Salivary Glands; Signal Transduction

2002
Expression of guanylin is downregulated in mouse and human intestinal adenomas.
    Biochemical and biophysical research communications, 2000, Jun-24, Volume: 273, Issue:1

    Guanylin is a pro-secretory hormone that is expressed in intestinal epithelia. Previously, we mapped the guanylin gene to mouse and human chromosomal regions containing multiple intestinal tumor-modifying loci. Here, we investigate whether guanylin expression is downregulated in precancerous human and mouse intestinal adenomas and whether diminished guanylin expression increases adenoma susceptibility in an animal model of intestinal cancer, the multiple intestinal neoplasia (Min) mouse. In situ hybridization analysis indicated diminished guanylin expression in both mouse and human adenomas. Northern analysis of mouse intestinal tissues showed strain-specific levels of guanylin expression but no correlation with the resistance or susceptibility of each strain to adenoma formation. Similarly, cDNA sequence analysis indicated no inactivating mutations or polymorphisms common to either the high or low adenoma-risk groups. Nonetheless, we have shown that significant loss of guanylin RNA in adenomas of mouse and human is a marker of intestinal epithelial cell transformation.

    Topics: Adenoma; Alleles; Animals; Colonic Neoplasms; Disease Models, Animal; DNA Mutational Analysis; Down-Regulation; Epithelial Cells; Gastrointestinal Hormones; Gene Expression Regulation, Neoplastic; Genes, APC; Genetic Predisposition to Disease; Humans; In Situ Hybridization; Intestinal Neoplasms; Jejunum; Mice; Mice, Inbred Strains; Mice, Mutant Strains; Mutation; Natriuretic Peptides; Peptides; Polymorphism, Genetic; Precancerous Conditions; RNA, Messenger; Species Specificity

2000