guanosine-triphosphate and Viremia

guanosine-triphosphate has been researched along with Viremia* in 2 studies

Other Studies

2 other study(ies) available for guanosine-triphosphate and Viremia

Article	Year
Identification of an NTPase motif in classical swine fever virus NS4B protein. Virology, 2011, Mar-01, Volume: 411, Issue:1 Classical swine fever (CSF) is a highly contagious and often fatal disease of swine caused by CSF virus (CSFV), a positive-sense single-stranded RNA virus within the Pestivirus genus of the Flaviviridae family. Here, we have identified conserved sequence elements observed in nucleotide-binding motifs (NBM) that hydrolyze NTPs within the CSFV non-structural (NS) protein NS4B. Expressed NS4B protein hydrolyzes both ATP and GTP. Substitutions of critical residues within the identified NS4B NBM Walker A and B motifs significantly impair the ATPase and GTPase activities of expressed proteins. Similar mutations introduced into the genetic backbone of a full-length cDNA copy of CSFV strain Brescia rendered no infectious viruses or viruses with impaired replication capabilities, suggesting that this NTPase activity is critical for the CSFV cycle. Recovered mutant viruses retained a virulent phenotype, as parental strain Brescia, in infected swine. These results have important implications for developing novel antiviral strategies against CSFV infection. Topics: Adenosine Triphosphate; Amino Acid Sequence; Amino Acid Substitution; Animals; Binding Sites; Catalytic Domain; Classical Swine Fever; Classical Swine Fever Virus; Conserved Sequence; Guanosine Triphosphate; Hydrolysis; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutant Proteins; Nucleoside-Triphosphatase; Sequence Alignment; Swine; Viral Load; Viral Nonstructural Proteins; Viremia; Virulence	2011
Cytomegalovirus cultures during maintenance DHPG therapy for cytomegalovirus (CMV) retinitis in acquired immunodeficiency syndrome (AIDS). Journal of medical virology, 1990, Volume: 30, Issue:1 Nine patients with acquired immunodeficiency syndrome (AIDS) and cytomegalovirus (CMV) retinitis on maintenance therapy with ganciclovir: 9(1,3-dihydroxy-2-propoxymethyl) guanine (DHPG) at high dose (30 mg/kg/week) or low dose (20 mg/kg/week) were tested every 1-2 weeks for CMV isolation from blood, saliva, and urine. Duration of therapy ranged from 1.5 to 12 months (average 5.3 months). During pretreatment and low-dose and high-dose maintenance therapy, CMV was isolated from 48/59 (81%), 90/211 (43%), and 40/290 (14%) of specimens, respectively. Three patients with progressive retinitis had viraemia more frequently than did six patients with stable retinitis, CMV being isolated from 29/47 (62%) and 17/121 (14%) of blood samples, respectively. Topics: Acquired Immunodeficiency Syndrome; Adult; Cytomegalovirus; Cytomegalovirus Infections; Guanosine Triphosphate; Humans; Male; Middle Aged; Prevalence; Retinitis; Viremia	1990

Article

Year

Identification of an NTPase motif in classical swine fever virus NS4B protein.

Virology, 2011, Mar-01, Volume: 411, Issue:1

Classical swine fever (CSF) is a highly contagious and often fatal disease of swine caused by CSF virus (CSFV), a positive-sense single-stranded RNA virus within the Pestivirus genus of the Flaviviridae family. Here, we have identified conserved sequence elements observed in nucleotide-binding motifs (NBM) that hydrolyze NTPs within the CSFV non-structural (NS) protein NS4B. Expressed NS4B protein hydrolyzes both ATP and GTP. Substitutions of critical residues within the identified NS4B NBM Walker A and B motifs significantly impair the ATPase and GTPase activities of expressed proteins. Similar mutations introduced into the genetic backbone of a full-length cDNA copy of CSFV strain Brescia rendered no infectious viruses or viruses with impaired replication capabilities, suggesting that this NTPase activity is critical for the CSFV cycle. Recovered mutant viruses retained a virulent phenotype, as parental strain Brescia, in infected swine. These results have important implications for developing novel antiviral strategies against CSFV infection.

Topics: Adenosine Triphosphate; Amino Acid Sequence; Amino Acid Substitution; Animals; Binding Sites; Catalytic Domain; Classical Swine Fever; Classical Swine Fever Virus; Conserved Sequence; Guanosine Triphosphate; Hydrolysis; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutant Proteins; Nucleoside-Triphosphatase; Sequence Alignment; Swine; Viral Load; Viral Nonstructural Proteins; Viremia; Virulence

2011

Cytomegalovirus cultures during maintenance DHPG therapy for cytomegalovirus (CMV) retinitis in acquired immunodeficiency syndrome (AIDS).

Journal of medical virology, 1990, Volume: 30, Issue:1

Nine patients with acquired immunodeficiency syndrome (AIDS) and cytomegalovirus (CMV) retinitis on maintenance therapy with ganciclovir: 9(1,3-dihydroxy-2-propoxymethyl) guanine (DHPG) at high dose (30 mg/kg/week) or low dose (20 mg/kg/week) were tested every 1-2 weeks for CMV isolation from blood, saliva, and urine. Duration of therapy ranged from 1.5 to 12 months (average 5.3 months). During pretreatment and low-dose and high-dose maintenance therapy, CMV was isolated from 48/59 (81%), 90/211 (43%), and 40/290 (14%) of specimens, respectively. Three patients with progressive retinitis had viraemia more frequently than did six patients with stable retinitis, CMV being isolated from 29/47 (62%) and 17/121 (14%) of blood samples, respectively.

Topics: Acquired Immunodeficiency Syndrome; Adult; Cytomegalovirus; Cytomegalovirus Infections; Guanosine Triphosphate; Humans; Male; Middle Aged; Prevalence; Retinitis; Viremia

1990