guanosine-triphosphate and Uterine-Cervical-Neoplasms

Infection with human papillomavirus (HPV) is relatively common and certain high-risk HPV strains can induce epithelial dysplasia, increasing the risk of cervical cancer. Green tea polyphenol (GTP) preparations exhibit diverse anti-inflammatory, antioxidative, and antitumor properties In Vitro and In Vivo. Topical GTP application has been recommended as a treatment for genital warts, but the effect of GTP treatment on HPV infection and HPV-associated cancer remains to be established. The present study aimed to explore the mechanism by which GTP affected HPV type 16 (HPV-16)-positive immortalized human cervical epithelial cells. Survival, apoptosis, and autophagocytosis of these cells following GTP treatment was assessed using CCK-8 assay, flow cytometry, and monodansylcadaverine (MDC) staining. These cells were further transfected with an shRNA specific for Nrf2 to generate stable Nrf2-knockdown cells. The levels of Caspase-3, Bcl-2, Bax, P53, Rb, HPV-16 E6, HPV-16 E7, P62, Beclin1 and LC3B were determined via Western blotting. These analyses revealed that GTP treatment induced autophagy and apoptosis in HPV-16-positive cells, while Nrf2 gene knockdown reversed GTP-induced autophagic and apoptotic effects. Together, these results suggested that GTP could alleviate HPV infection and HPV-associated precancerous lesions In Vitro by regulating the Nrf2 pathway, highlighting the therapeutic potential of GTP in treating HPV infection.

Topics: Apoptosis; Autophagy; Epithelial Cells; Female; Guanosine Triphosphate; Human papillomavirus 16; Humans; NF-E2-Related Factor 2; Oncogene Proteins, Viral; Papillomavirus E7 Proteins; Papillomavirus Infections; Polyphenols; Tea; Uterine Cervical Neoplasms

The colchicine-binding protein in procine-brain tissue is a dimer of molecular weight 110,000 that is believed to be the subunit of neuronal microtubules. Conditions are established under which the dimers aggregate with reproducible kinetics. This aggregation reaction, which is monitored by development of turbidity, has the following characteristics: (a) Colchicine inhibits development of turbidity; (b) the reaction inhibited by colchicine is reversed by long-wave ultraviolet irradiation; (c) the aggregation is temperature-dependent; (d) the reaction is nucleotide triphosphate-specific, being stimulated by 1 mM GTP; (e) the reaction appears to be specific for microtubule subunits since in the presence of other added proteins and in curde cell extracts, only microtubule subunits aggregate. On the basis of these criteria, we conclude that we have established an in vitro system for the aggregation of microtubule subunits that shares some of the properties characteristic of the in vivo assembly of cytoplasmic and spindle microtubules.

Topics: Animals; Brain; Cell Aggregation; Cell Differentiation; Cell Transformation, Neoplastic; Colchicine; Cytoplasm; Depression, Chemical; Electrophoresis, Polyacrylamide Gel; Female; Guanosine Triphosphate; In Vitro Techniques; Microtubules; Nerve Tissue Proteins; Nitrosourea Compounds; Nucleotides; Polymers; Protein Binding; Radiation Effects; Rats; Rats, Inbred Strains; Swine; Temperature; Time Factors; Ultraviolet Rays; Uterine Cervical Neoplasms