guanosine-triphosphate and Glomerulonephritis

Using the adenylate cyclase assay after Ross we examined hormone sensitivity of isolated glomerular cells. cAMP production was increased 1.3--1.6-fold by stimulation with isoproterenol, 1.5--1.8 times by prostaglandin E1 and 1.4--1.5 times by histamine. The isoproterenol reaction could be completely inhibited by propranolol, the histamine effect was abolished by the H2-blocking agent cimetidine. As control we applied sodium fluoride, which directly activates the catalytic adenylate cyclase unit, increasing the activity 1.8--2.7 times (depending on the method of homogenization). These findings could reflect some physiological or pathophysiological implications, which are discussed in the present report.

Topics: Adenylyl Cyclases; Animals; Cell Membrane; Cyclic AMP; Enzyme Activation; Glomerulonephritis; Guanosine Triphosphate; Histamine; Histamine Antagonists; In Vitro Techniques; Isoproterenol; Kidney Glomerulus; Prostaglandins E; Rats