guanosine-pentaphosphate and Poultry-Diseases

Salmonella Pullorum is a pathogen specific to birds that can cause Pullorum disease in young chickens and lead to considerable economic losses in the poultry industry. During transmission and infection, S. Pullorum will encounter various environmental stresses and host defenses. The stringent response is an important adaptation response induced by (p)ppGpp, and in Salmonella, (p)ppGpp is synthesized by two (p)ppGpp synthetases, RelA and SpoT. To investigate the role of (p)ppGpp synthetases in the adaptation and pathogenicity of S. Pullorum, a (p)ppGpp synthetases mutant (ΔrelAΔspoT) was constructed, and its physiological phenotypes and pathogenicity, as well as transcription profiling, were compared with the parent strain. The ΔrelAΔspoT mutant showed decreased ability to form biofilms, and reduced resistance to acidic, alkaline, high osmolarity and H

Topics: Animals; Bacterial Proteins; Biofilms; Chickens; Gene Deletion; Guanosine Pentaphosphate; Mice; Poultry Diseases; RAW 264.7 Cells; Salmonella; Salmonella Infections, Animal; Specific Pathogen-Free Organisms; Virulence; Virulence Factors