guanosine-diphosphate and HIV-Infections

guanosine-diphosphate has been researched along with HIV-Infections* in 2 studies

Other Studies

2 other study(ies) available for guanosine-diphosphate and HIV-Infections

ArticleYear
HIV-1 requires Arf6-mediated membrane dynamics to efficiently enter and infect T lymphocytes.
    Molecular biology of the cell, 2011, Apr-15, Volume: 22, Issue:8

    As the initial barrier to viral entry, the plasma membrane along with the membrane trafficking machinery and cytoskeleton are of fundamental importance in the viral cycle. However, little is known about the contribution of plasma membrane dynamics during early human immunodeficiency virus type 1 (HIV-1) infection. Considering that ADP ribosylation factor 6 (Arf6) regulates cellular invasion via several microorganisms by coordinating membrane trafficking, our aim was to study the function of Arf6-mediated membrane dynamics on HIV-1 entry and infection of T lymphocytes. We observed that an alteration of the Arf6-guanosine 5'-diphosphate/guanosine 5'-triphosphate (GTP/GDP) cycle, by GDP-bound or GTP-bound inactive mutants or by specific Arf6 silencing, inhibited HIV-1 envelope-induced membrane fusion, entry, and infection of T lymphocytes and permissive cells, regardless of viral tropism. Furthermore, cell-to-cell HIV-1 transmission of primary human CD4(+) T lymphocytes was inhibited by Arf6 knockdown. Total internal reflection fluorescence microscopy showed that Arf6 mutants provoked the accumulation of phosphatidylinositol-(4,5)-biphosphate-associated structures on the plasma membrane of permissive cells, without affecting CD4-viral attachment but impeding CD4-dependent HIV-1 entry. Arf6 silencing or its mutants did not affect fusion, entry, and infection of vesicular stomatitis virus G-pseudotyped viruses or ligand-induced CXCR4 or CCR5 endocytosis, both clathrin-dependent processes. Therefore we propose that efficient early HIV-1 infection of CD4(+) T lymphocytes requires Arf6-coordinated plasma membrane dynamics that promote viral fusion and entry.

    Topics: ADP-Ribosylation Factor 6; ADP-Ribosylation Factors; CD4-Positive T-Lymphocytes; Endocytosis; Female; Gene Silencing; Guanosine Diphosphate; HEK293 Cells; HeLa Cells; HIV Infections; HIV-1; Humans; Membrane Fusion; Microscopy, Fluorescence; Phosphatidylinositol 4,5-Diphosphate; Receptors, CCR5; Receptors, CXCR4; RNA, Small Interfering; Transfection; Vesiculovirus; Virus Internalization; Virus Replication

2011
Changes in purine nucleotide content in the lymphocyte subpopulations of patients infected with HIV.
    Clinica chimica acta; international journal of clinical chemistry, 1994, Volume: 225, Issue:2

    Topics: Adenine; Adenosine Diphosphate; Adenosine Triphosphate; Adult; CD4-Positive T-Lymphocytes; Female; Guanine; Guanosine Diphosphate; Guanosine Triphosphate; HIV Infections; HIV Seropositivity; HIV-1; Humans; Lymphocyte Subsets; Male; Middle Aged; Purine Nucleotides; T-Lymphocyte Subsets

1994