guanosine-5--o-(2-thiodiphosphate) and Inflammation

Lipopolysaccharide (LPS) is widely recognized as a potent activator of monocytes/macrophages, and its effects include an altered production of key mediators, such as inflammatory cytokines and chemokines. The involvement of G. We report here that GOT-loaded liposomes inhibited activation of MAPK and blocked the production of the cytokines IL1β, TNFα, IL6, and MCP1 induced by LPS in monocytes and macrophages. Moreover, GOT encapsulated in liposomes reduced monocyte adhesion and chemotaxis. All demonstrated events were in contrast with free GOT, which showed reduced or no effect on monocyte/macrophage activation with LPS.. This study demonstrates the potential of liposomal GOT in blocking LPS proinflammatory effects in monocytes/macrophages.

Topics: Cells, Cultured; Chemokines; Cytokines; GTP-Binding Protein alpha Subunits, Gi-Go; Guanosine Diphosphate; Humans; Inflammation; Interleukin-1beta; Lipopolysaccharides; Liposomes; Macrophage Activation; Macrophages; Monocytes; Thionucleotides; Tumor Necrosis Factor-alpha