griseofulvin and Shock

Teratogenic effect of two mitotic poisons, griseofulvin and colchicine, was confirmed. A similar effect of another antimitotic agent, vinblastin, was demonstrated. The teratogenic effect of these poisons is expressed as a reduction of ommatidia in adult flies when the drug is fed to larvae. The highest frequency of phenocopies was recorded in temperature- and mutagen-sensitive strains. The mutagenic activity of vinblastin and griseofulvin was confirmed by the wing-spot test (somatic mutation and recombination test, SMART) in Drosophila melanogaster. In addition, this test demonstrated mutagenic activity of colchicine. All of the mitotic poisons induced small single spots but did not increase frequency of twin spots mwh/flr. Spot frequency was significantly higher in mutagen-sensitive mutants having defective excision repair. Heat shock (45-min exposure at 37 degrees C) decreased the frequency of phenocopies induced by the mitotic poisons. When third-instar larvae were subjected to heat shock prior to drug administration, the frequency of mutant cell clones was significantly reduced. These results indicate participation of heat-shock proteins in the protection of microtubules in actively proliferating cells of D. melanogaster.

Topics: Abnormalities, Drug-Induced; Adaptation, Physiological; Animals; Colchicine; DNA Repair; Drosophila melanogaster; Griseofulvin; Hot Temperature; Larva; Mitosis; Mutagens; Recombination, Genetic; Shock; Vinblastine

Efficiency of heat shock and three compounds belonging to a group of mitotic poisons (colchicine (Cl), griseofulvin (Gf) and vinblastine (Vb)) in induction of dominant lethal mutations (DLM) in the Canton S wild type strain and the l(l)ts403 temperature-sensitive strain was shown. The mutagen-sensitive strain, mus(2)201Gl, displays no sensitivity to these drugs according to the DLM criterion. Heat shock followed by the Vb and Gf treatment leads to a reduction in the frequencies of DLM. Conversely, a combination of Cl with heat shock increases the yield of DLM.

Topics: Animals; Colchicine; Drosophila melanogaster; Embryo, Nonmammalian; Female; Genes, Lethal; Griseofulvin; Hot Temperature; Male; Microtubules; Mitosis; Mutation; Shock; Species Specificity; Vinblastine

Modification of the teratogenic effect of griseofulvin by hyperthermia was demonstrated in line Canton-S of Drosophila melanogaster. Heat shock (37 degrees C for 45 min) during II instar larvae significantly decreased the frequency of chemomorphoses that phenocopy ey mutation (eyeless, 4:2.0).

Topics: Animals; Drosophila melanogaster; Female; Griseofulvin; Hot Temperature; Larva; Male; Mutation; Shock; Teratogens