griseofulvin and Inflammation

Topics: Adolescent; Antifungal Agents; Child; Econazole; Griseofulvin; Humans; Inflammation; Male; Tinea Capitis; Trichophyton

Topics: Administration, Oral; Adolescent; Antifungal Agents; Diagnosis, Differential; Erythema; Griseofulvin; Humans; Inflammation; Male; Scalp; Tinea Capitis; Ultrasonography

Mesoporous silicon particles hold great potential in improving the solubility of otherwise poorly soluble drugs. To effectively translate this feature into the clinic, especially via oral or parenteral administration, a thorough understanding of the interactions of the micro- and nanosized material with the physiological environment during the delivery process is required. In the present study, the behaviour of thermally oxidized porous silicon particles of different sizes interacting with Caco-2 cells (both non-differentiated and polarized monolayers) was investigated in order to establish their fate in a model of intestinal epithelial cell barrier. Particle interactions and TNF-α were measured in RAW 264.7 macrophages, while cell viabilities, reactive oxygen species and nitric oxide levels, together with transmission electron microscope images of the polarized monolayers, were assessed with both the Caco-2 cells and RAW 264.7 macrophages. The results showed a concentration and size dependent influence on cell viability and ROS-, NO- and TNF-α levels. There was no evidence of the porous nanoparticles crossing the Caco-2 cell monolayers, yet increased permeation of the loaded poorly soluble drug, griseofulvin, was shown.

Topics: Animals; Caco-2 Cells; Cell Death; Cell Membrane Permeability; Cell Survival; Epithelial Cells; Griseofulvin; Humans; Inflammation; Intestines; Intracellular Space; Macrophages; Mice; Nanoparticles; Nitric Oxide; Oxidation-Reduction; Porosity; Reactive Oxygen Species; Silicon; Solubility; Temperature; Tumor Necrosis Factor-alpha

This protocol describes microsphere-based protease assays for use in flow cytometry and high-throughput screening. This platform measures a loss of fluorescence from the surface of a microsphere due to the cleavage of an attached fluorescent protease substrate by a suitable protease enzyme. The assay format can be adapted to any site or protein-specific protease of interest and results can be measured in both real time and as endpoint fluorescence assays on a flow cytometer. Endpoint assays are easily adapted to microplate format for flow cytometry high-throughput analysis and inhibitor screening.

Topics: Animals; Biotinylation; Flow Cytometry; Fluorescence Resonance Energy Transfer; Green Fluorescent Proteins; High-Throughput Screening Assays; Humans; Inflammation; Kinetics; Microspheres; Peptide Hydrolases; Peptides; Reproducibility of Results; Temperature

A series of new fluorinated coumarins and 1-aza coumarins have been synthesized and the presence of fluorine in these molecules and its effect on their anti-microbial, anti-inflammatory and analgesic activities are discussed. The results of bioassay showed that these newly synthesized compounds containing fluorine exhibit moderate analgesic and excellent anti-inflammatory and potential anti-bacterial and anti-fungal activities, compared to the other halogenated compounds. All the newly synthesized compounds were characterized by elemental analysis, IR, 1H NMR, 13C NMR, 19F NMR, EI-MS, and FAB-MS. The ORTEP diagram of one of the compounds is reported herein.

Topics: Analgesics; Animals; Aza Compounds; Bacteria; Coumarins; Drug Design; Fluorine; Fungi; Inflammation; Mice

The effect of occlusion and griseofulvin on the duration and severity of acute dermatophytosis was assessed in guinea pigs. Sixty guinea pigs given standard Trichophyton mentagrophytes infections were divided into 5 groups: (A) control, (B) intermittent wet occlusion, (C) continuous-wet occlusion, (D) griseofulvin, (E) griseofulvin-continuous-wet occlusion. Lesions were largest in the control group, smallest in the griseofulvin-continuous-occlusion group, and of intermediate area in the other groups. The degree of inflammation and alopecia was not affected by occlusion, but was markedly reduced or eliminated with griseofulvin. Healing of the lesions occurred more rapidly in the griseofulvin-continuous-occlusion group than in any other group. This study suggests that either occlusion of the inoculated site or oral griseofulvin markedly reduces the expected area of the fungal lesion, and occlusion together with griseofulvin shortens the duration of the lesion.

Topics: Animals; Female; Griseofulvin; Guinea Pigs; Humans; Inflammation; Male; Occlusive Dressings; Time Factors; Tinea; Trichophyton

Topics: Animals; Female; Griseofulvin; Histiocytes; Histocytochemistry; Inflammation; Leukocytes; Lipids; Liver; Male; Mice; Necrosis

Topics: Anti-Bacterial Agents; Antibiotics, Antitubercular; Dermatologic Agents; Griseofulvin; Inflammation; Pharmacology; Rheumatic Diseases; Rheumatology

Topics: Animals; Animals, Laboratory; Anti-Inflammatory Agents; Griseofulvin; Inflammation