glycogen and Sarcoma

Topics: Fatty Acids, Nonesterified; Gluconeogenesis; Glycogen; Humans; Hypoglycemia; Insulin Antagonists; Insulin-Like Growth Factor II; Liver; Prognosis; Sarcoma; Surgical Procedures, Operative

Topics: Adenofibroma; Adenoma, Pleomorphic; Alkaline Phosphatase; Atrophy; Basement Membrane; Breast; Breast Diseases; Breast Neoplasms; Carcinoma; Carcinoma, Adenoid Cystic; Carcinosarcoma; Endocrine Glands; Epithelial Cells; Epithelium; Female; Glycogen; Humans; Hyperplasia; Lipids; Male; Middle Aged; Muscle, Smooth; Myoepithelioma; Myofibrils; Pigments, Biological; Salivary Gland Neoplasms; Sarcoma; Sweat Glands; Water

Ewing family of tumors (EFTs) comprise highly malignant, nearly undifferentiated neoplasms including Ewing's sarcoma (ES), primitive neuroectodermal tumor (PNET) and a spectrum of other unusual variants. In general, EFTs are included among small blue cell tumors. Establishing histological diagnosis can be difficult; CD99 and FLI1 immunohistochemical staining has improved diagnosis, but these markers are not specific for EFTs. The diagnosis of EFTs is confirmed by molecular diagnostic testing showing the presence of established rearrangements of the EWS gene. The use of this molecular signature in EFTs revealed rare variants in the histomorphologic spectrum of these tumors. The authors report an unusual variant of EFT in the mandible of a 17-year-old patient, which was confirmed by translocation rearrangement in EWR1 gene at 22q12 by fluorescence in situ hybridization. The unusual histologic features, with prominent spindling of tumor cells and deviation from the classic features of Ewing's sarcoma posed a diagnostic challenge for the medical centers involved in the diagnosis and treatment of this patient. This highlights the importance of the genetic study of undifferentiated sarcomas to identify rare morphologic variants of ES, in view of the chemosensitivity of EFTs and how this affects patient management.

Topics: 12E7 Antigen; Adolescent; Antigens, CD; Carboxylesterase; Cell Adhesion Molecules; Chondrosarcoma, Mesenchymal; Chromosomes, Human, Pair 22; Diagnosis, Differential; Female; Follow-Up Studies; Gene Rearrangement; Glycogen; Humans; In Situ Hybridization, Fluorescence; Mandibular Neoplasms; RNA-Binding Protein EWS; Sarcoma; Sarcoma, Ewing; Translocation, Genetic

A clear cell sarcoma that developed on the left back of a 19-year-old Japanese female was studied ultrastructurally. The findings included basal laminae, desmosomelike junctions, cytoplasmic interdigitations, lamellar bodies, and immature melanosomes. These findings suggest schwannian differentiation of this tumor, since they are not usual features of malignant melanoma except for melanosomes. Therefore this tumor seems to be more akin to malignant schwannoma than to malignant melanoma.

Topics: Adult; Cell Differentiation; Female; Glycogen; Humans; Melanoma; Microscopy, Electron; Sarcoma; Schwann Cells; Soft Tissue Neoplasms

A clinico-pathological, light microscopic and immunohistochemical study of 15 clear-cell sarcomas, with an ultrastructural analysis of 6 of the tumors, is presented. The tumors showed a strong predilection for tendons and aponeuroses of the extremities in predominantly young and middle-aged people. The clinical setting, course and light microscopic appearance agree well with the original description by Enzinger (1965). Nine of the 15 patients developed metastases, most of them including lymph nodes, and 8 of the patients had died at the time of follow-up (median follow-up time 4.8 years). Reducing pigment was demonstrated within the cells of 2 tumors. Ultrastructurally the 6 tumors studied had a uniform appearance with characteristically rounded or oval tumor cells with a single nucleus containing one or two very prominent nucleoli, a light-staining cytoplasm with a moderate amount of organelles and a variable content of glycogen. Polymorphic melanosomes were seen in the cells of one of the tumors. External laminas enclosed groups of tumor cells and invested parts of individual tumor cells. With immunoperoxidase analysis for S-100 protein positive staining was observed in the vast majority of the tumor cells of all 15 clear-cell sarcomas. Metastases appearing in 9 of the 15 cases showed positive staining for S-100 protein. There was a strong staining of the cytoplasm and generally a weak and varying staining of nuclei. The immunohistochemical and electron microscopic findings indicate that clear-cell sarcoma is a homogenous entity among soft tissue sarcomas, of probable neural crest derivation.

Topics: Adolescent; Adult; Age Factors; Aged; Extremities; Female; Glycogen; Histocytochemistry; Humans; Immunoenzyme Techniques; Lymphatic Metastasis; Male; Melanocytes; Microscopy, Electron; Middle Aged; Nerve Tissue Proteins; S100 Proteins; Sarcoma; Soft Tissue Neoplasms; Tendons

A further case of a spindle cell sarcoma of the pulmonary trunk is reported, with extension to the pulmonary valve and lungs. Some spindle cells contained glycogen and their cell walls acid mucosubstances. Electron microscopy showed two types of cell. The more common were polyhedral with poorly arranged fibrils in the cytoplasm and an abundant fuzzy coat. About 5 per cent of the cells contained neurosecretory granules.

Topics: Cardiovascular System; Digestive System; Endoplasmic Reticulum; Glycogen; Golgi Apparatus; Humans; Lung Neoplasms; Macrophages; Male; Mast Cells; Middle Aged; Mitochondria; Pulmonary Artery; Pulmonary Valve; Sarcoma

A study of 40 cases of malignant round-cell tumour of one was made from the files of the Cancer Research Campaign's Bone Tumour Panel. Five pathologists made a careful study of observer error, involving repeated examination of routine paraffin sections, to determine whether the cases were a homogeneous group or a collection of differing sub-groups. Cell outline, nuclear staining, nuclear pleomorphism, conspicuous nucleoli, reticulin pattern and intracellular glycogen were the histological features selected for study. For each feature, the results were analysed to assess the importance of differences between tumours, between samples of tissue from the same tumour, and between observers. It is concluded that round-cell tumours of bone are a heterogeneous group, although completely distinct sub-groups could not be identified. Certain histological features tend to be associated, and it is reasonable to distinguish on histological grounds between Ewing's sarcoma and reticulum-cell sarcoma, although some tumours are not typical of either group.

Topics: Age Factors; Analysis of Variance; Bone Neoplasms; Cell Nucleolus; Cell Nucleus; Glycogen; Humans; Reticulin; Sarcoma

Cytologic and cytochemical examination of eighteen cases of round-cell sarcoma of bone allowed classification of these tumors into four cytologic groups. Additional cytochemical examinations based on the PAS and D-PAS reactions, and the demonstration of the activity of peroxidase, naphtol-ASD-Chloracetate esterase, alpha-naphthylacetate esterase, naphthol-AS-acetate esterase with and without sodium fluoride inhibition, acid and alkaline phosphatases yielded no evidence of uniform behavior among the individual groups or within any single group. The studies showed that a positive glycogen reaction cannot be used as a basic criterion for the classification of such tumors as Ewing's sarcoma and for regarding them as a uniform tumor group. It is possible that a pool of tumors is involved, including tumors of monocytic and probably of lymphocytic origin, reticulum-cell sarcoma, tumors of myelocytic and erythroplastic origin, stem-cell tumors, and endothelial-cell tumors. Histologic examination alone is not sufficient for the classification of round-cell sarcomas of bone, and it should be supplemented by cytologic and cytochemical or histochemical methods. Osteosarcomas (23 cases) and chondrosarcomas (8 cases) display cells which are characteristic for these tumors and which could be correlated with their benign counterparts, osteoblasts and chondroid cells. The histologically recognizable degree of malignancy of chondrosarcoma can be evaluated better with the cytologic than with the histologic technic. Indications of the possibilities of differential diagnosis based on the cytologic pictures of benign and malignant osteoplastic and chondroplastic tumors, giant-cell tumors and chordoma are discussed.

Topics: Adolescent; Bone Neoplasms; Cell Nucleolus; Cell Nucleus; Child; Chondroblastoma; Chondroma; Chondrosarcoma; Chordoma; Cytoplasm; Female; Giant Cell Tumors; Glycogen; Humans; Infant; Male; Naphthol AS D Esterase; Osteosarcoma; Periodic Acid-Schiff Reaction; Phosphoric Monoester Hydrolases; Sarcoma; Sarcoma, Ewing

Topics: Basement Membrane; Capillaries; Cell Membrane; Cytoplasmic Granules; Endoplasmic Reticulum; Glycogen; Golgi Apparatus; Humans; Inclusion Bodies; Intercellular Junctions; Lipofuscin; Mitochondria; Neoplasms, Glandular and Epithelial; Paraganglioma, Extra-Adrenal; Sarcoma

Topics: Adult; Female; Glycogen; Histocytochemistry; Humans; Neoplasm Recurrence, Local; Sarcoma; Tendons

Topics: Adipose Tissue; Cartilage; Chondrosarcoma; Chordoma; Connective Tissue; Female; Fibroma; Fibrosarcoma; Glycogen; Hemangioma; Humans; Leiomyoma; Leiomyosarcoma; Lipoma; Liposarcoma; Muscle, Smooth; Neoplasms, Connective Tissue; Neoplasms, Muscle Tissue; Neoplasms, Nerve Tissue; Neurilemmoma; Neurofibroma; Sarcoma; Sarcoma, Synovial; Synovial Membrane; Uterus

Topics: Alkaline Phosphatase; Alkylating Agents; Animals; Antineoplastic Agents; Aziridines; Busulfan; Carcinoma, Ehrlich Tumor; Dihydrolipoamide Dehydrogenase; DNA; DNA, Neoplasm; Electron Transport Complex IV; Glycogen; Histocytochemistry; Lipids; Lymphoma, Non-Hodgkin; Mammary Neoplasms, Animal; Mammary Neoplasms, Experimental; Melanoma; Mice; Neoplasms; Neoplasms, Experimental; Nitrogen Mustard Compounds; Pharmacology; Research; RNA; RNA, Neoplasm; Sarcoma; Sarcoma, Experimental; Succinate Dehydrogenase; Tissue Culture Techniques

Topics: Animals; Ascites; Autoradiography; Carcinoma, Ehrlich Tumor; DNA; DNA Replication; DNA, Neoplasm; Glucose; Glycogen; Glycolysis; Metabolism; Research; Sarcoma; Sarcoma, Yoshida; Thymidine

Topics: Carbohydrate Metabolism; Electrons; Glycogen; Glycogenolysis; Histocytochemistry; Microscopy; Microscopy, Electron; Phosphorylase Kinase; Rats; Research; Sarcoma; Sarcoma, Yoshida

Topics: Ascites; Electrons; Glycogen; Glycogen Phosphorylase, Muscle Form; Microscopy, Electron; Mitochondria; Phosphorylase Kinase; Phosphorylases; Rats; Research; Sarcoma; Sarcoma, Yoshida

Topics: Animals; Antineoplastic Agents; Glycogen; Glycogenolysis; Neoplasms; Sarcoma; Sarcoma, Yoshida

Thirty GRCH/15 tumors (a 1, 2, 5, 6-dibenzanthracene-induced chicken sarcoma) were examined in the light and the electron microscope. Associated with the sarcoma were two types of abnormal intranuclear lesions, one in the form of a vacuole, the other as an aggregate containing glycogen. In the electron microscope, one type of lesion observed showed an organized microfibrillar structure. Abnormal cytoplasmic formations occurred as massed clusters of thread-like or tubular material, which gave rise to small bodies with concentric shell structure; similar bodies were found associated with vacuoles.

Topics: Animals; Chickens; Cytoplasm; Electrons; Glycogen; Microscopy; Microscopy, Electron; Neoplasms, Experimental; Sarcoma; Vacuoles

Topics: Bone and Bones; Diagnosis, Differential; Glycogen; Humans; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Neoplasms; Sarcoma; Sarcoma, Ewing

Topics: Bone and Bones; Bone Neoplasms; Glycogen; Humans; Neoplasms; Sarcoma

Topics: Animals; Glycogen; Glycogenolysis; Liver; Liver Glycogen; Mice; Neoplasms; Sarcoma

Topics: Animals; Ascites; Carcinoma, Ehrlich Tumor; Glycogen; Neoplasms; Sarcoma; Sarcoma, Experimental; Sarcoma, Yoshida