glycogen and Parotid-Neoplasms

A case of multifocal nodular oncocytic hyperplasia (MNOH) of the bilateral parotid gland is presented. An 80-year-old woman was admitted to hospital because of painless swellings in bilateral parotid regions. Histologically, the nodular lesion had incomplete capsules and engulfed the surrounding parotid gland at the periphery. The lesions were mostly composed of clear cells, while the peripheries of the lesions had typical oncocytic cells with abundant fine granules. The histological existence of the clear cell component in the lesions led to misdiagnoses of other clear cell neoplasms. However, this case had multiple nodules in bilateral glands. No evidence of malignant histological findings was found. Moreover, the clear cells, as well as the oncocytic cells, were demonstrated to have mitochondria and glycogen in their cytoplasm using special staining. Based on these findings, the diagnosis of this case was MNOH in the parotid gland. We also discuss the differential diagnosis for clear cell lesions.

Topics: Adenoma, Oxyphilic; Aged, 80 and over; Diagnosis, Differential; Female; Glycogen; Humans; Hyperplasia; Mitochondria; Oxyphil Cells; Parotid Diseases; Parotid Gland; Parotid Neoplasms; Treatment Outcome

Nosologically, the designation clear cell oncocytoma seems illogical and contradictory when the definition of an oncocytoma relies on mitochondrial-rich cells; its relationship to the granular (mitochondrial-rich) oncocytoma needs further clarification. Electron microscopy of six examples of oncocytoma from five patients allowed assessment of cellular features in three clear cell variants, two typical oncocytomas and one with a roughly equal proportion of clear and granular tumor cells. Ultrastructurally, in the clear cell types of oncocytoma, a considerable to extensive portion of the central cytoplasm was occupied by monoparticulate glycogen with margination of mitochondria and other organelles. The degree of extraction of the glycogen during fixation and processing accounts for variation in the extent of the clear cell component as phosphotungstic acid-hematoxylin staining reveals considerable mitochondria in many of the tumor cells in the clear cell variant of oncocytoma. Ultrastructural findings further support an interrelationship between clear and granular cells, as some typical oncocytes are evident in the clear cell variant of oncocytoma, and one oncocytoma in this series had a roughly equal number of glycogen-rich and mitochondrial-rich tumor cells both of which took part in the formation of microlumens. The designation clear cell oncocytoma is appropriate within the oncocytoma subgroup of salivary gland tumors.

Topics: Adenoma, Oxyphilic; Glycogen; Granular Cell Tumor; Humans; Microscopy, Electron; Mitochondria; Parotid Neoplasms

A case of glycogen-rich adenocarcinoma of the parotid gland of a 23 years old man was investigated ultrastructually. The cytoplasm of the tumour cells was abundantly filled with glycogen-rich particles. Among these particles well developed cytoplasmic organelles were observed, too. In addition, another tumour all type could be found, i.e. a tumour cell of basal cell origin with tonofibris in the cytoplasm, attached to the glycogen-rich tumour cell type by desmosomes.

Topics: Adenocarcinoma; Adult; Carcinoma, Basal Cell; Glycogen; Humans; Male; Parotid Neoplasms

Five cases of mucoepidermoid carcinoma of the parotid gland were studied ultrastructurally. In addition to epidermoid cells (tonofilament-rich) and mucous cells, three forms of intermediate cells were identified: mitochondria-rich cells, glycogen-rich cells, and ribosome-rich cells. Transitional forms of all cell types were observed. Ultrastructurally, cells rich in glycogen appeared to be the "clear cells" by light microscopy; cells rich in ribosomes appeared basophilic under light microscopy. Mitochondria-rich cells were lightly eosinophilic under light microscopy.

Topics: Adolescent; Adult; Aged; Carcinoma; Desmosomes; Female; Glycogen; Humans; Male; Microvilli; Middle Aged; Mitochondria; Parotid Neoplasms; Ribosomes

A quantitative electron microscopic study was performed in a case of parotid oncocytoma. The mean nuclear radius was estimated to be 3.4 mu, and the mean nuclear volume 171 cu mu. The mean cell volume was calculated to be 958 cu mu. The mitochondrial volume density value averaged 59% of the cytoplasmic volume, and the mean nuclear volume density was 24% of the cell volume. These findings are compared with previous quantitative electron microscopic data on various epithelial cells from the digestive system, including the salivary glands.

Topics: Adenoma; Aged; Cytoplasm; Female; Glycogen; Histocytochemistry; Humans; Mitochondria; Parotid Gland; Parotid Neoplasms

Electron microscopical study of an oncocytoma of the parotid gland disclosed the presence of two cell types, typical oncocytes and condensed oncocytes. The typical oncocyte contains abundant mitochondria that were tightly packed almost completely filling the entire cytoplasm. Only small areas were left for the remaining cytoplasmic organelles. The nuclei in these typical onocytes appeared oval or spheroid. Condensed oncocytes were scattered singly or in nests in tumorous tissue. These cells were also replete with mitochondria, but differed, however, in that many mitochondria showed evidence of degeneration and fusion. Nuclei of these cells were irregular, dense, and contained inclusions and glycogen granules. The relationship of nuclear envelopes to mitochondria and other cellular components has been described previously, but to our knowledge, this represents an original finding in the case of an oncocytoma.

Topics: Adenoma; Cell Nucleus; Connective Tissue; Cytoplasm; Cytoplasmic Granules; Desmosomes; Endoplasmic Reticulum; Glycogen; Histocytochemistry; Humans; Lysosomes; Male; Microscopy, Electron; Middle Aged; Mitochondria; Parotid Gland; Parotid Neoplasms

Topics: Adult; Age Factors; Aged; Carcinoma; Carcinoma, Adenoid Cystic; Diagnosis, Differential; Endoplasmic Reticulum; Epithelial Cells; Female; Glycogen; Humans; Male; Middle Aged; Myoepithelioma; Parotid Neoplasms; Pinocytosis; Salivary Gland Neoplasms; Sex Factors; Submandibular Gland

Topics: Adenoma; Cytoplasmic Granules; Electron Transport Complex IV; Female; Glycogen; Histocytochemistry; Humans; Microscopy, Electron; Middle Aged; Mitochondria; Parotid Neoplasms

Topics: Adenocarcinoma; Adenoma; Aged; Carcinoma, Squamous Cell; Diagnosis, Differential; Female; Glycogen; Humans; Parotid Neoplasms

Topics: Adenoma; Aged; Cytoplasm; Female; Glycogen; Humans; Microscopy, Electron; Middle Aged; Mitochondria; Parotid Neoplasms

Topics: Glycogen; Glycosaminoglycans; Histocytochemistry; Humans; Mucins; Neoplasms, Germ Cell and Embryonal; Neuraminic Acids; Parotid Neoplasms

Topics: Adenoma; Glycogen; Humans; Parotid Neoplasms; Salivary Gland Neoplasms; Salivary Glands