glycogen and Papilloma

The development of hepatocellular tumors was investigated with histological, histochemical, and morphometrical methods in male Sprague-Dawley rats continuously administered N-nitrosodiethylamine (DEN) or N-nitrosomorpholine (NNM) in the drinking water at low doses (0.5 mg DEN/100 ml; 1 mg NNM/100 ml). Groups of control, DEN-, and NNM-treated rats were investigated at 5-week intervals. Similar results were obtained in DEN- and NNM-treated rats. Two types of areas composed of basophilic or glycogenotic hepatocytes were observed preceding the appearance of hepatocellular adenomas and carcinomas. Besides their cytologic differences, the basophilic and glycogenotic areas induced displayed distinct histochemical features. Both types of areas were detected simultaneously and increased in parallel with time to a similar incidence, but basophilic areas reached larger sizes than the glycogenotic ones. Furthermore, each type of area, which clustered around and along efferent veins, was differently linked to tumorigenesis. Basophilic areas frequently developed into basophilic adenomas and trabecular carcinomas through a characteristic sequence. Early basophilic areas consisted of hepatocytes with lamellar cytoplasmic hyperbasophilia and exhibited the normal laminar liver structure. With time, an increasing number of basophilic areas also contained hepatocytes with powdered diffuse hyperbasophilia, which frequently were arranged in thick trabeculae, showed abundant mitotic figures, and invaded efferent veins. Neither such signs of malignancy nor conversion into basophilic areas or tumors could be established for areas of clear and acidophilic glycogenotic hepatocytes. However, a few small glycogenotic adenomas probably developed from glycogenotic areas. Our data thus underline the central role of basophilic areas for hepatocarcinogenesis. Moreover, taking into account the data from other experiments, it seems likely that although glycogenotic areas may be associated with the application of some carcinogens at high doses, they are not obligatory precursors of hepatocellular tumors.

Topics: Adenoma; Administration, Oral; Animals; Carcinogens; Diethylnitrosamine; Dose-Response Relationship, Drug; Esophageal Neoplasms; Glycogen; Histocytochemistry; Incidence; Liver; Liver Neoplasms, Experimental; Lung Neoplasms; Male; Nitrosamines; Papilloma; Rats

We report a new case of a clear-cell acanthoma with an unusual location (para-anal), which was studied by electronmicroscopic and immuno-histological methods, using monoclonal antibodies. The results of this study showed that: a) the inflammatory cellular infiltrate comprised a small number of T-suppressor/cytotoxic lymphocytes and a small number of Langerhans cells, b) Langerhans cells were present in the affected epidermis in reduced numbers (2.2 p. 100) when compared to normal epidermis (4-6 p. 100), c) there was a disorder of both keratinization and epidermal differentiation as was shown by the abnormal reactivity of the tumor to monoclonal antibodies KL1 and BL7, d) human papilloma viruses do not seem to be involved in the genesis of the tumour, e) tumoral cells did not show (secretory) eccrine differentiation, f) the pemphigus antigen was present in the intercellular space of the affected epidermis.

Topics: Anal Canal; Antibodies, Monoclonal; Fluorescent Antibody Technique; Glycogen; Humans; Male; Middle Aged; Papilloma; Skin; Skin Neoplasms

The choroid plexus papillomas are rare neoplasms. They constitute about 0.5 per 100 of all intracranial tumors. From a histological point of view they are generally benign tumors. However they give a high mortality rate due to their location near vital structures, the rapid production of intracranial hypertension, and the rich vascularity making risky the surgical treatment.

Topics: Cerebral Ventricle Neoplasms; Choroid Plexus; Glycogen; Humans; Organoids; Papilloma

Two patients with clear-cell acanthoma with multiple lesions are reported; histologic and histochemical findings are similar to previous descriptions. The ultrastructural study confirms the overload of glycogen in keratinocytes, associated with an increase of mitochondria and nuclear deformations. The abundance of Langerhans' cells is emphasized. Extrusion of glycogen by keratinocytes and its phagocytosis by Langerhans' cells is suggested.

Topics: Aged; Female; Glycogen; Histocytochemistry; Humans; Langerhans Cells; Middle Aged; Papilloma; Skin Neoplasms; Steroids

The inter-relationships of 32 pathologic and 7 clinical parameters encountered in the study of 1000 examples of invasive breast carcinoma have been presented. In some instances the biological significance of these associations is at present unclear. In others it is to be noted that there is no information provided as to the rank of their significance. Nevertheless, the associations that were encountered not only help further characterize the various forms of breast cancer but also provide information regarding the possible biological significance of some of their features. Although it is not our intention to minimize the possible significance of the inter-relationships of pathologic parameters, most emphasis in the summarizing statements which follow has been placed upon those correlations which may relate to prognosis. In this regard reference has been made to short-term treatment failure, vis a vis local recurrence and/or metastases, which may not necessarily accurately reflect patient survival, although generally such a relationship exists. Information in this regard as well as to the rank of the significance of these pathologic features shall be forthcoming when sufficient time has elapsed since the inception of this study to allow for such conclusions, i.e. survival or long-term treatment failure rates. Lastly, it becomes evident that the guidelines followed in the examination of these specimens appear to represent at least the minimum requirements necessary for a meaningful pathologic evaluation of breast carcinoma.

Topics: Adenocarcinoma, Mucinous; Adult; Blood Vessels; Breast Neoplasms; Calcium; Carcinoma; Carcinoma, Adenoid Cystic; Carcinoma, Intraductal, Noninfiltrating; Carcinoma, Papillary; Carcinosarcoma; Cell Nucleus; Connective Tissue; Elastic Tissue; Female; Glycogen; Histiocytes; Humans; Lymphatic Metastasis; Lymphocytes; Middle Aged; Mucins; Necrosis; Neoplasms, Multiple Primary; Paget's Disease, Mammary; Papilloma; Sweat Glands

Topics: Glycogen; Humans; Papilloma; Skin Neoplasms

Topics: Adult; Aged; Basement Membrane; Biopsy; Carcinoma, Basal Cell; Diagnosis, Differential; Facial Neoplasms; Female; Glycogen; Hair; Humans; Male; Middle Aged; Papilloma; Sex Factors; Skin Neoplasms; Warts

Topics: Cerebral Ventricle Neoplasms; Child, Preschool; Choroid Plexus; Cytoplasm; Female; Glycogen; Humans; Infant; Microscopy, Electron; Middle Aged; Papilloma

Topics: Acetates; Chondroitin; Colon; Colonic Neoplasms; Glycogen; Histocytochemistry; Humans; Hyaluronic Acid; Intestinal Mucosa; Intestinal Polyps; Keto Acids; Neuraminic Acids; Papilloma; Periodic Acid; Polysaccharides; Staining and Labeling; Sulfates

Topics: Adolescent; Adult; Aged; Basement Membrane; Cell Division; Cell Nucleus; Child; Cilia; Cytoplasm; Epithelium; Female; Glycogen; Humans; Inclusion Bodies; Keratins; Male; Middle Aged; Mucus; Nasal Mucosa; Nasal Septum; Neoplasm Recurrence, Local; Nose; Nose Neoplasms; Papilloma; Paranasal Sinus Neoplasms; Paranasal Sinuses

Topics: Bronchial Diseases; Bronchial Neoplasms; Carcinoma, Bronchogenic; Carcinoma, Squamous Cell; Diagnosis, Differential; Glycogen; Histocytochemistry; Humans; Lung Neoplasms; Papilloma; Transferases

Topics: Biopsy; Breast; Breast Diseases; Breast Neoplasms; Endoplasmic Reticulum; Female; Glycogen; Humans; Microscopy, Electron; Microtomy; Osmium; Papilloma; Staining and Labeling

Topics: Glycogen; Histocytochemistry; Humans; Male; Mast Cells; Microscopy, Electron; Middle Aged; Neutrophils; Papilloma; Skin Neoplasms

Topics: Cell Nucleolus; Cell Nucleus; Cytoplasm; DNA, Neoplasm; Glycogen; Histocytochemistry; Humans; Lipids; Neoplasm Proteins; Papilloma; Polysaccharides; RNA, Neoplasm; Urinary Bladder Diseases; Urinary Bladder Neoplasms

Topics: Astrocytoma; Brain Neoplasms; Ependymoma; Ganglioneuroma; Glioblastoma; Glycogen; Histocytochemistry; Humans; Medulloblastoma; Neurilemmoma; Oligodendroglioma; Papilloma; Tuberous Sclerosis

Topics: Connective Tissue; Epithelium; Glycogen; Glycosaminoglycans; Histocytochemistry; Humans; Mucous Membrane; Papilloma; Ureter; Ureteral Neoplasms

Topics: Carcinoma; Carcinoma, Papillary; Connective Tissue; Epithelium; Glycogen; Glycosaminoglycans; Histocytochemistry; Humans; Papilloma; Ureteral Neoplasms

Topics: Adenoma, Sweat Gland; Adult; Aged; Diagnosis, Differential; Electron Transport Complex IV; Female; Glucosyltransferases; Glycogen; Histocytochemistry; Humans; Male; Middle Aged; Papilloma; Skin Neoplasms; Succinate Dehydrogenase; Tyrosine Decarboxylase

Topics: Alkaline Phosphatase; Animals; Benz(a)Anthracenes; Coloring Agents; DNA, Neoplasm; Glycogen; Histocytochemistry; Keratoacanthoma; Neoplasms, Experimental; Papilloma; Rabbits; RNA, Neoplasm; Sulfhydryl Compounds