glycogen and Neurilemmoma

Topics: Adenosine Diphosphate; Adenosine Monophosphate; Adenosine Triphosphate; Aerobiosis; Anaerobiosis; Animals; Brain; Brain Neoplasms; Creatine; Ependymoma; Glioma; Glucose; Glycogen; Glycolysis; Humans; Hypoxia; Ischemia; Lactates; Meningioma; Mice; Neoplasms, Experimental; Neurilemmoma; Oxygen Consumption; Periodic Acid; Phosphofructokinase-1; Phosphorus; RNA; Vestibulocochlear Nerve

Corpora amylacea (CA) are inclusions with starch-like composition that occur in various conditions. We have observed CA in gastrointestinal leiomyomas (GILM) and hypothesised that they differ from intracytoplasmic hyaline globules (HG) of GILM. We aimed to investigate the anatomical distribution, prevalence, staining characteristics and ultrastructural features of CA and compare them with HG of GILM.. Slides from a consecutive series of resected GILM and bland spindle cell tumours were examined for CA and HG. Special stains, electron microscopy and elemental analysis were performed on select leiomyomas.. CA occurred in 13/35 GILM (37%) from the following sites: oesophagus (4/8), stomach (5/7) including one frozen section, small intestine (1/2) and large intestine (3/18), but were not identified in 19 gastrointestinal stromal tumours (12 gastric, 7 small intestinal; p=0.0019), five schwannomas (three gastric, two small intestinal; p=0.154) and 35 non-GILM (p=0.0001). The densities of CA ranged from one per 4-200 mm(2). CA stained intensely with periodic acid Schiff after diastase predigestion (PASD), Alcian blue and ubiquitin, and faintly in peripheral zones for desmin and smooth muscle actin. Ultrastructurally, CA consisted of an electron-dense outer layer and a fibrillar core with scattered particle matter. HG were present in all leiomyomas, but showed variable staining for PASD, negative staining for Alcian blue and ubiquitin, and positive staining for smooth muscle markers.. CA are a distinctive histological feature of approximately one third of GILM with different composition to HG. These differences may represent divergent degenerative processes or different stages of a single degenerative process over time.

Topics: Biomarkers, Tumor; Desmin; Female; Gastrointestinal Neoplasms; Gastrointestinal Stromal Tumors; Gastrointestinal Tract; Glycogen; Humans; Hyalin; Intermediate Filaments; Leiomyoma; Male; Microscopy, Electron; Middle Aged; Neurilemmoma

A malignant epithelioid schwannoma occurred on the right second toe of a 30-year-old Japanese man. It was a firm, flesh-colored, benign-appearing nodule and measured 13 x 9 mm in diameter and 6 mm in height. To our knowledge, this is the first case of malignant epithelioid schwannoma occurring on the toe. Histopathology was characterized by a circumscribed nodule in the dermis that predominantly consisted of atypical large epithelioid cells with some spindle cells whose proliferation was similar to that of the Verocay bodies seen in ordinary schwannoma. Fontana-Masson staining demonstrated no melanin pigment in the tumor at the light microscopic level. The eosinophilic cytoplasm contained abundant glycogen and was positive for S-100 protein and HMB-45, as usually seen in melanomas. Electron microscopy revealed that there was an abundance of long-spacing collagen in the extracellular matrix, and the cells contained numerous dense-cored granules. But no definite melanosomes were observed in any stage. As far as we are aware, this is the first case of a malignant epithelioid schwannoma showing HMB-45 positivity.

Topics: Adult; Antigens, Neoplasm; Cytoplasm; Epidermis; Foot Diseases; Glycogen; Humans; Male; Neurilemmoma; Organelles; S100 Proteins; Skin Neoplasms; Toes; Vimentin

Biopsies from 15 human gliomas, five meningiomas, four Schwannomas, one medulloblastoma, and four normal brain areas were analyzed for 12 enzymes of energy metabolism and 12 related metabolites and cofactors. Samples, 0.01-0.25 microgram dry weight, were dissected from freeze-dried microtome sections to permit all the assays on a given specimen to be made, as far as possible, on nonnecrotic pure tumor tissue from the same region. Great diversity was found with regard to both enzyme activities and metabolite levels among individual tumors, but the following generalities can be made. Activities of hexokinase, phosphorylase, phosphofructokinase, glycerophosphate dehydrogenase, citrate synthase, and malate dehydrogenase levels were usually lower than in brain; glycogen synthase and glucose-6-phosphate dehydrogenase were usually higher; and the averages for pyruvate kinase, lactate dehydrogenase, 6-phosphogluconate dehydrogenase, and beta-hydroxyacyl coenzyme A dehydrogenase were not greatly different from brain. Levels of eight of the 12 enzymes were distinctly lower among the Schwannomas than in the other two groups. Average levels of glucose-6-phosphate, lactate, pyruvate, and uridine diphosphoglucose were more than twice those of brain; 6-phosphogluconate and citrate were about 70% higher than in brain; glucose, glycogen, glycerol-1-phosphate, and malate averages ranged from 104% to 127% of brain; and fructose-1,6-bisphosphate and glucose-1,6-bisphosphate levels were on the average 50% and 70% those of brain, respectively.

Topics: Adolescent; Adult; Aged; Brain; Brain Neoplasms; Child; Child, Preschool; Energy Metabolism; Female; Glioma; Glycogen; Glycolysis; Humans; Male; Medulloblastoma; Meningioma; Middle Aged; Mitochondria; Neurilemmoma; Oxidative Phosphorylation

Topics: Adenosine Diphosphate; Adenosine Monophosphate; Adenosine Triphosphate; Adolescent; Adult; Aged; Brain Neoplasms; Child; Creatine Kinase; Energy Metabolism; Female; Glioma; Glycogen; Humans; Lipids; Male; Meningioma; Middle Aged; Neurilemmoma; Phosphates; Phosphocreatine; Uridine Triphosphate

Topics: Acid Phosphatase; Alkaline Phosphatase; Dihydrolipoamide Dehydrogenase; Esterases; Glucuronidase; Glycerolphosphate Dehydrogenase; Glycogen; Glycoside Hydrolases; Histocytochemistry; Humans; Hydrolases; In Vitro Techniques; Isocitrate Dehydrogenase; Lipid Metabolism; Malate Dehydrogenase; Neurilemmoma; Oxidoreductases; Succinate Dehydrogenase

Topics: Adipose Tissue; Cartilage; Chondrosarcoma; Chordoma; Connective Tissue; Female; Fibroma; Fibrosarcoma; Glycogen; Hemangioma; Humans; Leiomyoma; Leiomyosarcoma; Lipoma; Liposarcoma; Muscle, Smooth; Neoplasms, Connective Tissue; Neoplasms, Muscle Tissue; Neoplasms, Nerve Tissue; Neurilemmoma; Neurofibroma; Sarcoma; Sarcoma, Synovial; Synovial Membrane; Uterus

Topics: Cell Membrane; Cerebellar Neoplasms; Cytosol; Glycogen; Humans; Microscopy, Electron; Mitochondria; Nerve Degeneration; Neurilemmoma; Neurofibrils; Reticular Formation; Spinal Cord Neoplasms

Topics: Astrocytoma; Brain Neoplasms; Ependymoma; Ganglioneuroma; Glioblastoma; Glycogen; Histocytochemistry; Humans; Medulloblastoma; Neurilemmoma; Oligodendroglioma; Papilloma; Tuberous Sclerosis