glycogen and Myeloproliferative-Disorders

Topics: Acid Phosphatase; Alkaline Phosphatase; Bone Marrow Diseases; Bone Neoplasms; Esterases; Glucosyltransferases; Glycogen; Histocytochemistry; Leukemia; Lipid Metabolism; Myeloproliferative Disorders; Peroxidases; Succinate Dehydrogenase

In platelets of patients affected with myeloproliferative disorders, glycolytic and glycogenolytic flux have been examined. Results of studies on glucose uptake, glycogen breakdown and lactate formation have been reported. No difference in glucose uptake between controls and patients was observed, but in patients a higher lactate formation was always noted in the presence or absence of exogenous glucose, in correlation with a higher glycogen breakdown rate. Results could be explained by modified regulation mechanisms on glycogen phosphorylase.

Topics: Adult; Aged; Blood Platelets; Female; Glucose; Glycogen; Glycolysis; Humans; Lactates; Male; Middle Aged; Myeloproliferative Disorders

Native and functioning platelets in patients with primary myelofibrosis and myeloproliferative syndrome were examined by transmission electron microscopy. Among various types of ultrastructural abnormalities in the platelets, three features were emphasized: hypoplasia of the surface connecting system (SCS) with few orifices, hyperplasia of the dense tubular system, and considerable variety in numbers of granule. Morphological analysis was made on platelets of these patients and of normal subjects. Functional morphology of the abnormal platelets was examined in the aggregate samples either by ADP or collagen and the effluent blood from platelet retention test. The abnormal platelets were more or less indifferent from the aggregates and underwent only to a slight degree the changes observed in normal platelets. They frequently retained their native, round, smooth-surfaced form without pseudpod, and did not show the inward shift of organelles; furthermore, they retained more granules than did normal platelets. From these morphological findings we speculated that these ultrastructurally abnormal platelets had an impaired release reaction as a result of the dysfunction of SCS in passing released substances, of the inability of microfilaments to constrict in expelling these substances and sometimes because of a lack of granules as the sources.

Topics: Adenosine Diphosphate; Blood Platelets; Cell Membrane; Collagen; Cytoplasmic Granules; Glycogen; Golgi Apparatus; Humans; Lupus Erythematosus, Systemic; Microscopy, Electron; Microtubules; Mitochondria; Myeloproliferative Disorders; Platelet Aggregation; Primary Myelofibrosis; Stimulation, Chemical

Topics: Anemia; Blood Platelet Disorders; Blood Platelets; Glycogen; Hematologic Diseases; Humans; Myeloproliferative Disorders

Topics: Blood Platelets; Diabetes Mellitus; Glycogen; Glycogen Storage Disease; Histocytochemistry; Humans; Infant, Newborn; Infant, Newborn, Diseases; Infant, Premature, Diseases; Leukemia; Leukemia, Lymphoid; Leukemia, Myeloid; Liver Cirrhosis; Microscopy, Phase-Contrast; Myeloproliferative Disorders; Polycythemia; Primary Myelofibrosis; Purpura, Thrombocytopenic; Remission, Spontaneous; Uremia; von Willebrand Diseases

Topics: Acids; Bone Marrow Diseases; Diagnosis, Differential; Glycogen; Humans; Leukemia, Myeloid; Myeloproliferative Disorders; Peroxidases; Polycythemia Vera; Primary Myelofibrosis

Topics: Anemia; Bone Marrow Diseases; Glycogen; Histocytochemistry; Humans; Megakaryocytes; Methods; Myeloproliferative Disorders; Periodic Acid; Staining and Labeling

Topics: Bone Marrow Diseases; Child; Child, Preschool; Clinical Enzyme Tests; Glucosyltransferases; Glycogen; Humans; Leukemia, Lymphoid; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Leukocytes; Myeloproliferative Disorders

Topics: Blood Platelets; Clinical Enzyme Tests; Glycogen; Humans; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; Myeloproliferative Disorders; Polycythemia; Primary Myelofibrosis