glycogen and Multiple-Myeloma

Topics: Adult; Aged; Alkaline Phosphatase; Female; Glycogen; Histocytochemistry; Humans; Lipids; Male; Middle Aged; Multiple Myeloma; Neutrophils; Peroxidases

This study presents evidence on the occurrence of an inverse relationship between activation of glycogen synthetase and inactivation of phosphorylase in a platelet preparation in vitro. The activities of glycogen synthetase and phosphorylase and the pattern of changes in these activities in platelets from controls and multiple myeloma patients were compared. Platelets obtained from multiple myeloma patients were shown to have an increased glycogen content, accompanied by an elevated level of glycogen synthetase a and a decreased activity of phosphorylase a. The pattern of changes in these enzyme activities during incubation was also different in platelets of controls and multiple myeloma patients. Extracts from patients' platelets prevented glycogen synthetase activation and phosphorylase inactivation of control platelets. Preincubation of platelets from multiple myeloma patients in control plasma resulted in an increased rate of glycogen synthetase activation, and abolished activation of phosphorylase which was found after preincubation in autologous plasma.

Topics: Adult; Aged; Blood Platelets; Enzyme Activation; Female; Glycogen; Glycogen Synthase; Humans; Male; Middle Aged; Multiple Myeloma; Phosphorylases

Topics: Alkaline Phosphatase; Biopsy; Bone Cysts; Bone Diseases; Bone Neoplasms; Chondroma; Chondrosarcoma; Clinical Enzyme Tests; Diagnosis, Differential; Femoral Neoplasms; Fibrosarcoma; Giant Cell Tumors; Glycogen; Hemangioma; Hemangiosarcoma; Histocytochemistry; Humans; Ilium; Lymphoma, Large B-Cell, Diffuse; Multiple Myeloma; Neoplasm Metastasis; Osteoma; Osteoma, Osteoid; Osteomyelitis; Osteosarcoma; Ribs; Sarcoma, Ewing; Tibia; Trephining; Tuberculosis, Osteoarticular

The lymphocyte glycogen content has been measured semi-quantitatively by staining peripheral blood films by the periodic-acid-Schiff (P.A.S.) technique. The high values reported in chronic lymphatic leukaemia, lymphosarcoma, Hodgkin's disease, and infectious mononucleosis have been confirmed. High values have also been found in cases with chronic suppuration, ulcerative colitis, myelomatosis, and epithelial malignancies. Our results suggest that a high lymphocyte glycogen content may be produced by several different mechanisms and that the test is of little value in differential diagnosis.

Topics: Colitis, Ulcerative; Diagnosis, Differential; Glycogen; Hodgkin Disease; Humans; Leukemia, Lymphocytic, Chronic, B-Cell; Lymphocytes; Multiple Myeloma; Staining and Labeling