glycogen and Mouth-Diseases

Histological and ultrastructural features of 25 oral snuff dipper's lesions with distinctive subepithelial hyaline deposits were investigated. Periodic acid-Schiff reaction with and without diastase digestion demonstrated the presence of glycogen and other carbohydrates, but histochemical stains for normal collagen, elastin and fibrin showed a weak variable reactivity of the deposit. Although in 7/25 cases the deposit was in close proximity to labial salivary glands and on occasions ducts were found within the deposit, the presence of mucin was not a consistent feature. Congo red staining and immunohistochemical investigation with an anti-amyloid antibody did not support the previous contention that such deposits were amyloid in nature. Immunohistochemically, collagen antibodies also provided negative results, but ultrastructural features of three biopsies studied suggest that the bulk of this deposit is made up of collagen, as typical cross-striated fibrils were found. The pathogenesis of this deposit could therefore be interpreted as over-production and/or reduced turnover of collagen by resident fibroblasts, which is further altered by the ingredients of toombak. The deposit does not appear to be a secretory product.

Topics: Adult; Amylases; Amyloid; Antibodies; Basement Membrane; Biopsy; Carbohydrates; Collagen; Coloring Agents; Congo Red; Elastin; Epithelium; Fibrin; Fibroblasts; Glycogen; Humans; Hyalin; Middle Aged; Mouth Diseases; Mucins; Periodic Acid-Schiff Reaction; Plants, Toxic; Salivary Ducts; Salivary Glands; Sudan; Tobacco, Smokeless

Topics: Aged; Candida albicans; Carcinoma, Squamous Cell; Diagnosis, Differential; Epithelium; Glycogen; Humans; Keratins; Leukoplakia, Oral; Lichen Planus; Middle Aged; Mitosis; Mouth Diseases; Mouth Neoplasms

Forty-four oral lesions with epithelial dysplasia and 25 other benign and malignant lesions of the oral mucosa were examined after staining with hematoxylin-eosin and diastase controlled PAS. The intensity of the PAS-positivity for glycogen, the grade of dysplasia, the type of keratinization and the degree of subepithelial inflammation were recorded. Histologically normal epithelium at the margins of the lesions were used as controls. The presence and amount of glycogen in normal epithelium at the margins of the lesions were used as controls. The presence and amount of glycogen in normal epithelium at the margin of the lesions were used as controls. The presence and amount of glycogen in normal epithelium varied with the form of keratinization in that non- or parakeratinized epithelium was rich in glycogen whereas there was a negative glycogen-reaction in orthokeratinized epithelium. The most striking feature was an abrupt limitation of the glycogen at the junction between nondysplastic and dysplastic epithelium. The difference in the amount of glycogen in normal and dysplastic epithelium as assessed semiquantitatively, was statistically significant. There was no statistically significant difference in glycogen content with different degrees of dysplasia. The diastase controlled PAS-staining may therefore be a useful method of distinguishing dysplastic from nondysplastic epithelium in doubtful cases. Pseudoepitheliomatous hyperplastic epithelium covering granular cell myoblastoma did not contain any glycogen. Five of six squamous cell carcinomas and four verrucous carcinomas contained no demonstrable glycogen. Glycogen was present in the epithelium of the cases of lichen planus and "denture hyperplasia" investigated.

Topics: Adult; Aged; Carcinoma, Papillary; Carcinoma, Squamous Cell; Epithelial Attachment; Glycogen; Humans; Middle Aged; Mouth Diseases; Mouth Mucosa; Mouth Neoplasms; Staining and Labeling

Superficial, transitional and polygonal buccal epithelial cells from patients with the erosive type of lichen planus were compared with similar cells from non-keratinizing human buccal mucosa. Transitional cells from non-keratinizing buccal mucosa had glycogen lakes and membrane-coating granules with electron dense cores. Surface cells were not keratinized and pathological cells were less granular. In lichen planus membrane-coating granules were numerous, markedly electron dense, and some showed an internal lamellar structure. Surface cells were keratotic or parakeratotic. Processes of macrophages were associated with the pathological membrane-coating granules.

Topics: Cytoplasmic Granules; Epithelium; Gingiva; Glycogen; Intracellular Membranes; Keratins; Lichen Planus; Microscopy, Electron; Mouth Diseases; Mouth Mucosa; Organoids

The literature on the epithelial changes in oral submucous fibrosis is reviewed. The epithelial changes seen in thirty-eight biopsy specimens from thirty-eight patients with oral submucous fibrosis are presented in detail. It was seen that in submucous fibrosis there is a tendency toward epithelial atrophy associated with hyperothokeratosis and pyknotic changes in the nuclei of the basal-cell layer. Hyperplasia of the epithelium usually associated with hyperparakeratosis was also noticed. A striking feature in this study was the absence of glycogen from most of the Grade III (severe) cases. Vacuolization of prickle-cell layer, increased mitotic activity, and epithelial atypia were also noticed in a few cases. The significance of submucous fibrosis as a possible precancerous lesion is emphasized, with reference to the recent reports in the literature.

Topics: Atrophy; Epithelium; Glycogen; Mitosis; Mouth Diseases; Mouth Mucosa

Cytochemical studies of glycogen of oral mucosa cells have been made on the smears by freeze-drying and PAS staining. The specimens were obtained from different areas of oral cavity of 77 human subjects and an attempt was made to find some interrelation amoung glycogen deposition, keratinization and inflammation. The largest glycogen deposition was found in the mucosa cells from mouth floor and cheek, a little in those from gingiva and quite a small or no glycogen in those from mucosa of hard palate and tongue. In gingiva the cells showing much more keratinization were less in glycogen contents, and vice versa. In inflammation some increase in glycogen contents were found in the gingivitis and the highest glycogen content in the cases of denture irritation of the palate as far as the present observation is concerned.

Topics: Adolescent; Adult; Aged; Cheek; Denture, Complete; Female; Gingiva; Gingivitis; Glycogen; Humans; Inflammation; Keratins; Male; Middle Aged; Mouth Diseases; Mouth Floor; Mouth Mucosa; Palate; Stomatitis, Aphthous; Tongue

Topics: Basement Membrane; Biopsy; Connective Tissue; Disulfides; DNA; Epithelium; Glycogen; Glycosaminoglycans; Histocytochemistry; Humans; Microscopy, Electron; Mouth Diseases; Mouth Mucosa; RNA; Skin Diseases; Sulfhydryl Compounds; Tyrosine

Topics: Adult; Aged; Biopsy; Cytoplasmic Granules; Epithelium; Female; Gingiva; Glycogen; Histocytochemistry; Humans; Keratins; Leukoplakia; Leukoplakia, Oral; Lichen Planus; Male; Microscopy, Electron; Middle Aged; Mouth Diseases; Mouth Mucosa; Mouth Neoplasms; Palate; Tongue

Topics: Adolescent; Adult; Aged; DNA; Female; Glycogen; Glycosaminoglycans; Histocytochemistry; Humans; Leprosy; Male; Middle Aged; Mouth Diseases; Mouth Mucosa; RNA

Topics: Epithelium; Glycogen; Glycosaminoglycans; Histocytochemistry; Humans; Hyperplasia; Mast Cells; Mouth Diseases; Palate; Precancerous Conditions; Staining and Labeling

Topics: Acrylic Resins; Adult; Aged; Amino Acids; Chromium Alloys; Denture Bases; Female; Glycogen; Glycosaminoglycans; Histocytochemistry; Humans; Keratosis; Male; Middle Aged; Mouth Diseases; Mouth Mucosa

Topics: Face; Gastrointestinal Tract; Glycogen; Mouth Diseases