glycogen and Monieziasis

Lyo- and desmo-glycogen were estimated in relation to growth and maturity of proglottids of a cestode, Moniezia benedeni. Total and lyo-glycogen show an increasing trend from immature to mature region (i.e., from region 1 to 6) and the increased level seen in the 6th region is maintained up to the 9th region with a fall in the 10th (gravid) region. These results indicate that mature proglottids store more carbohydrate than immature and gravid proglottids. Desmo-glycogen showed no variation in different regions of the strobila. The percentage of lyo-glycogen to total carbohydrate reveals that most of the carbohydrate (85-93%) is stored in a labile form.

Topics: Animals; Cestoda; Glycogen; Intestine, Small; Monieziasis; Sheep; Sheep Diseases; Tissue Distribution

A particulate polysaccharide was isolated by differential centrifugation and alkali extraction from homogenates of the cestode Moniezia expansa. The polysaccharide had the structure of a glycogen. Its chemical properties, infra-red spectrum and optical rotation showed that it consisted of alpha-1,4- and alpha-1,6-linked glucopyranose units. Examination of the complex with iodine and the precipitate with concanavalin-A showed that the structure was highly branched. Oxidation with periodate and hydrolysis with alpha- and beta-amylase were used to measure mean chain lengths. For the particulate preparation the average chain length was 12-9 glucose units and the exterior and interior chain lengths were 9-0 and 2-9 units respectively. The particulate preparation had a very high sedimentation constant (s20, w = 910) with a smaller component at about s20, w = 600, but the alkali extracted material had an s20, w = 61 similar to that shown by alkali degradation of the particulate preparation. The morphology of the particulate material was similar to that of rat liver glycogen, alpha, beta- and possible gamma-particles being identified by electron microscopy. The alpha-particles were relatively stable under acidic conditions remaining intact down to pH 2-5. At pH 1-7 the alpha-particles dissociated into their constituent beta-particles with a consequent decrease in the opalescence of the solution. The nitrogen content of 0-9% was high for a glycogen.

Topics: Amylases; Animals; Cestoda; Glycogen; Hydrolysis; Microscopy, Electron; Molecular Weight; Monieziasis; Nitrogen; Spectrophotometry