glycogen and Lymphoma

Topics: 12E7 Antigen; Actins; Antigens, CD; Bone Neoplasms; CD3 Complex; Cell Adhesion Molecules; Child; Diagnosis, Differential; Diagnostic Errors; Glycogen; Humans; Immunohistochemistry; Intercellular Junctions; Leukocyte Common Antigens; Liability, Legal; Lymphoma; Microscopy, Electron; Neuroectodermal Tumors, Primitive; Pathology; Phosphopyruvate Hydratase; Sarcoma, Ewing; Vimentin

Activated natural killer (A-NK) cells, defined by immunophenotype and selected by adherence to the plastic, were cultured from murine splenocytes for up to 10 days with the addition of 1000 U/ml of recombinant human IL-2 at 48 h intervals. During culture days 2-4 with high DNA synthesis the initially non-granulated small cells established large granular lymphocyte (LGL) morphology and then differentiated further into giant hypergranulated cells with huge accumulations of glycogen. Timed EM observations indicated that specific dual-compartment (lytic) granules arose by a sequence of events starting with neo-synthesis of small progenitors with a dense core and a few membranous lamellae at one pole. Core and vesicular regions probably expanded independently to give the mature organization of the granule. Eventually, the vesicular region of granules contained large amounts of multi-lamellar material and probable debris, and the dense core could be multiplied. Intracellular proteoglycans, visualized with Cupromeronic Blue cytochemistry, were organized in a three-dimensional network within the dense cores. In contrast with earlier reports, and in spite of several-fold increased granularity, the in vitro cytotoxicity of the A-NK cells against YAC-1 and B16 cells decreased after the third day of culture. A-NK cells with glycogen accumulations caused focal clearing in melanoma monolayers whereas younger effectors adhered to the targets. It is concluded that high dose IL-2 stimulation causes more far-going progressive morphological and functional differentiations of the A-NK cells than has previously been observed with bearing for the use of these cells in experimental adoptive immunotherapy.

Topics: Animals; Cell Adhesion; Cell Communication; Cell Compartmentation; Cell Division; Cell Nucleus; Cytoplasmic Granules; Cytotoxicity, Immunologic; Dose-Response Relationship, Immunologic; Glycogen; Humans; Immunophenotyping; Inclusion Bodies; Interleukin-2; Killer Cells, Lymphokine-Activated; Lipid Metabolism; Lymphocyte Activation; Lymphoma; Lysosomes; Male; Melanoma, Experimental; Mice; Mice, Inbred C57BL; Proteoglycans; Time Factors; Tumor Cells, Cultured

Topics: Acid Phosphatase; Diagnosis, Differential; Glycogen; Histocytochemistry; Humans; Infectious Mononucleosis; Lymphadenitis; Lymphocytes; Lymphoma; Naphthol AS D Esterase; Tuberculosis, Lymph Node

The periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) technique, which has the same cytochemical significance as PAS staining of light microscopy, was undertaken to reveal the distribution of complex carbohydrates with vicinal glycols in human lymphocytes in several conditions at the ultra-structural level. The PA-TCH-SP method stained the clustered and the scattered cytoplasmic granules, Golgi apparatus and glycogen particles of lymphocytes. In the buffy coat, the lymphocytes with the clustered cytoplasmic granules contained less glycogen particles compared with the lymphocytes without the clustered cytoplasmic granules. The majority of T-lymphocytes separated from the venous blood possessed PA-TCH-SP positive clustered cytoplasmic granules, but glycogen particles were scanty or negligible, while B lymphocytes were rich with glycogen particles but had scanty clustered cytoplasmic granules. The T and B cell-derived cultured cell lines had a similar reactivity to the peripheral T and B cells.

Topics: B-Lymphocytes; Cell Line; Cell Separation; Cell Transformation, Viral; Cytoplasmic Granules; Glycogen; Golgi Apparatus; Herpesvirus 4, Human; Humans; Lymphoma; Microscopy, Electron; Periodic Acid; T-Lymphocytes

In human tumors (cancer of the liver, stomach, lymphogranulomatosis of lymphnodes, metastases in lymphnodes, ascites ovarian tumor cells) the authors studied the content of enzymes participating in glycogen biosynthesis through uridine diphosphateglucose mechanism--UDPG-glycogensynthetase, UDPG-pyrophosphroylase, phosphoglucomutase. As a rule, in human tumor cells the amount of glycogen and the activity of enzymes of its biosynthesis is decreased, the more the higher proliferative activity and autonomization of tumor cells.

Topics: Enzyme Activation; Glycogen; Glycogen Synthase; Humans; Liver Neoplasms; Lymphoma; Neoplasms; Nucleotidyltransferases; Phosphoglucomutase; Stomach Neoplasms; Uridine Diphosphate Glucose; Uridine Diphosphate Sugars; UTP-Glucose-1-Phosphate Uridylyltransferase

Topics: Agglutination; Agglutination Tests; Animals; Binding Sites, Antibody; Carbohydrates; Cell Line; Cell Membrane; Cell Transformation, Neoplastic; Concanavalin A; Cricetinae; Fibroblasts; Fluorescent Antibody Technique; Glycogen; Leukemia, Experimental; Leukemia, Myeloid, Acute; Lymphocytes; Lymphoma; Male; Mice; Movement; Rats; Tritium; Trypsin

Topics: Female; Glycogen; Histocytochemistry; Humans; Leukemia, Lymphoid; Lymphatic Diseases; Lymphocytes; Lymphoma; Male

Topics: Acid Phosphatase; Alkaline Phosphatase; Culture Techniques; Esterases; Glycogen; Humans; Lectins; Leukemia, Lymphoid; Lymphocytes; Lymphoma; Succinate Dehydrogenase

Topics: Acid Phosphatase; Culture Techniques; Esterases; Glycogen; Hodgkin Disease; Humans; Lectins; Leukemia; Leukemia, Lymphoid; Lymphocyte Activation; Lymphocytes; Lymphoma; Lymphoma, Non-Hodgkin; Succinate Dehydrogenase

Topics: Chemical Phenomena; Chemistry; Glycogen; Humans; Leukemia; Leukemia, Lymphoid; Lymphocytes; Lymphoma; Lymphoma, Non-Hodgkin; Microscopy; Microscopy, Phase-Contrast

Topics: Aminosalicylic Acid; Aminosalicylic Acids; Antibodies; Biochemical Phenomena; Biochemistry; Cell Biology; Culture Media; Glycogen; Growth; Leukemia; Leukemia, Lymphocytic, Chronic, B-Cell; Leukemia, Lymphoid; Leukocyte Count; Lymphocytes; Lymphoma; Lymphoma, Non-Hodgkin; Phytohemagglutinins

Topics: Alkaline Phosphatase; Blood Cells; Endocarditis; Endocarditis, Bacterial; Female; Glycogen; Hepatitis; Hepatitis A; Histocytochemistry; Humans; Infant, Newborn; Infectious Mononucleosis; Leukemia; Leukemia, Lymphoid; Leukemia, Myeloid; Lymphoma; Polycythemia Vera; Pregnancy; Pregnancy Complications; Pregnancy Complications, Infectious; Primary Myelofibrosis; Sepsis

Topics: Glycogen; Humans; Leukemia; Leukemia, Lymphoid; Lymphocytes; Lymphocytosis; Lymphoma; Lymphoma, Non-Hodgkin