glycogen and Infertility--Male

Triploid oysters have poor gonadal development, which can not only bring higher economic benefits but also have a potential application in the genetic containment of aquaculture. However, the key factors that influence germ cell development in triploid oysters remain unclear. In this study, data-independent acquisition coupled to transcriptomics was applied to identify genes/proteins related to sterility in triploid

Topics: Animals; Cell Division; Cell Proliferation; Crassostrea; Energy Metabolism; Female; Gene Expression Profiling; Gene Ontology; Germ Cells; Glycogen; Gonads; Infertility, Female; Infertility, Male; Male; Proteins; Proteomics; Reproducibility of Results; Transcription, Genetic; Triploidy

This study investigated the effects of Tetracarpidium conophorum leaf extract on infertility induced by ethanol in male rats. Thirty rats were randomly divided into six groups of five animals each: Group 1 (positive control) received 0.9% saline only; Group 2 (ethanol alone) were given only 30% ethanol orally at 7 ml/kg body weight per day, thrice in a week; groups 3, 4 and 5 were given ethanol and co-treated with 50, 500 and 1,000 mg/kg body weight of leaf extract, respectively, while Group 6 was given ethanol and co-treated with a fertility drug, clomiphene citrate. Ethanol treatment resulted in significant (p < .05) decrease in LDH activity, G-6PDH activity, glycogen content, 3β and 17β HSD activities and testicular and epididymal Zn and Se contents and furthermore decrease in testicular sperm count, viability and marked increment in total sperm abnormalities, rate of sperm analysis parameters and consequently decreased reproductive hormone levels. Interestingly, co-administration of ethanol with either T. conophorum extract or drug almost ameliorated the toxic assault imposed by ethanol on reproductive organs and improved seminal qualities of the rats.

Topics: Animals; Clomiphene; DNA Fragmentation; Ethanol; Euphorbiaceae; Glycogen; Infertility, Male; L-Lactate Dehydrogenase; Male; Oxidative Stress; Plant Extracts; Rats; Sperm Count; Sperm Motility; Spermatozoa; Testis

The ability of sterile males to survive, disperse, find, and mate with wild females is key to the success of sterile insect technique (SIT). The Release of Insects carrying a Dominant Lethal (RIDL) system is a genetics-based SIT strategy for Aedes aegypti. We examine two aspects of insect performance, flight potential (dispersal ability) and teneral energy reserves, by comparing wild-type (WT) males with genetically-modified lines carrying the tetracycline-repressible constructs OX513A and OX3604C. Our results show significant differences in the flight capacity of the modified lines. OX513A males bred with tetracycline covered 38% less distance, while OX3604C males reared without tetracycline spent 21% less time in flight than their WT counterparts. Such differences in flight performance should be considered when designing release programs (e.g., by placing release sites sufficiently close together to achieve adequate coverage). All mosquito lines had similar teneral carbohydrate contents, though males of the OX3604C line contained more lipids. The addition of tetracycline to the larval diet did not influence the flight potential of the males; however, it did change the teneral sugar reserves of the WT and the lipid reserves of both the WT and the OX3604C lines.

Topics: Aedes; Animal Distribution; Animals; Animals, Genetically Modified; Body Constitution; Carbohydrate Metabolism; Carbohydrates; Energy Metabolism; Female; Flight, Animal; Genes, Dominant; Genes, Lethal; Glycogen; Infertility, Male; Insect Vectors; Lipid Metabolism; Lipids; Male; Mosquito Control; Mutation; Tetracycline; Wings, Animal; Yellow Fever

Topics: Adult; Glycogen; Humans; Infertility, Male; Male; Sertoli Cells; Spermatogenesis; Testis

Topics: Glucosyltransferases; Glycogen; Histocytochemistry; Humans; Infertility, Male; Male; Testis