glycogen and Hyperkalemia

Topics: Acidosis, Renal Tubular; Adipose Tissue; Alkalosis; Animals; Antihypertensive Agents; Blood Volume; Clinical Trials as Topic; Diuretics; Erythrocytes; Ethacrynic Acid; Furosemide; Glucose; Glycogen; Humans; Hyperkalemia; Hypokalemia; Membrane Potentials; Potassium; Pyrazines; Sodium; Spironolactone; Triamterene

To investigate the myocardial protective effects of pinacidil-induced hyperpolarized arrest and compare with those afforded by conventional depolarized hyperkalemic arrest.. Eighteen dogs were equally divided into three groups: normothermic hyperpolarized group (Group A), hypothermic hyperpolarized group (Group B), and hyperkalemic group (Group C). Pinacidil (50 mumol/L) containing 37 degrees C St. Thomas solution (K+ 5 mmol/L, 10 ml/kg), pinacidil (50 mumol/L, Sigma, USA) containing 4 degrees C St. Thomas solution (K+ 5 mmol/L, 10 ml/kg) and 4 degrees C standard St. Thomas solution (K+ 16 mmol/L, 10 ml/kg) were infused respectively through the aortic root after aortic-clamping. Heart arrest and its recovery, ultrastructure of the myocardium, the level of serum myocardial enzymes, and lipid peroxide and adenine nucleotide of the myocardium were measured. Hemodynamics during ischemia and after reperfusion were observed.. The percentages of normal mitochondria and glycogen did not change much during ischemia (except at 60 min) and after reperfusion in B Group, but declined markedly in Group C 30 min and 60 min after ischemia and 20 min after reperfusion (P < 0.01). In Group A, they were lower than those of Group B before ischemia, but higher than those of Group C. The recoveries of CO, SV, CI, LVSW, RVSW and MAP in Group B were significantly better than those in other two groups 15 min and 30 min after reperfusion (P < 0.05 and 0.01, respectively). However, they were still better in Group A than those in Group C (P < 0.05 and 0.01, respectively). The onset of heart arrest was faster in Groups C and B than that in Group A. Highly elevated serum myocardial enzymes were observed 60 min after ischemia and 20 min after reperfusion in Group C, while they were only mild in the hyperpolarized groups, especially in Group B, and their recoveries were rapid. Adenine nucleotides of the myocardium were better preserved in Group B than in other two groups 30 min, 60 min after ischemia, and 20 min after reperfusion (P < 0.05 and 0.01, respectively). They were also much better in Group A than in Group C (P < 0.05 and 0.01, respectively). Lipid peroxide of the myocardium were significantly lower in Group B than in other groups 20 min after reperfusion (P < 0.01), and they were lower in Group A than in Group C (P < 0.05).. Myocardial protection for global ischemia during cardiopulmonary bypass (CPB) could be achieved with hyperpolarized heart arrest induced by pinacidil, an ATP-sensitive potassium channel opener, especially in the hypothermic state. The protection is weaker in normothermia but is still superior to that with traditional depolarized hyperkalemic arrest.

Topics: Adenine Nucleotides; Alkaline Phosphatase; Animals; Antihypertensive Agents; Cardiopulmonary Bypass; Creatine Kinase; Dogs; Glycogen; Heart; Heart Arrest, Induced; Hemodynamics; Hyperkalemia; L-Lactate Dehydrogenase; Lipid Peroxides; Mitochondria; Myocardial Reperfusion Injury; Myocardium; Phosphocreatine; Pinacidil; Time Factors

Topics: Acute Disease; Animals; Coronary Disease; Electrocardiography; Electrophysiology; Energy Metabolism; Glycogen; Heart; Hyperkalemia; Hypoxia; Membrane Potentials; Perfusion; Species Specificity; Swine

Topics: Ammonia; Animals; Bile; Bilirubin; Blood Glucose; Blood Pressure; Blood Proteins; Dogs; Fibrinogen; Glycogen; Hematocrit; Hemodynamics; Hepatectomy; Hydrogen-Ion Concentration; Hyperkalemia; Lactates; Liver Circulation; Liver Function Tests; Liver Transplantation; Perfusion; Portal System; Transaminases; Transplantation, Homologous; Urea

Topics: Adolescent; Adult; Biopsy; Diagnosis, Differential; Dilatation; Female; Femur; Glycogen; Histocytochemistry; Humans; Hyperkalemia; Lipids; Male; Mitochondria, Muscle; Muscles; Myofibrils; Paralysis; Potassium

Topics: Adolescent; Adult; Biopsy; Calcium; Carbohydrate Metabolism; Child; Child, Preschool; Chromosome Aberrations; Chromosome Disorders; Electromyography; Energy Transfer; Female; Glycogen; Humans; Hyperkalemia; Injections, Intravenous; Male; Microtomy; Muscle Contraction; Muscles; Myofibrils; Myotonia; Paralysis; Pedigree; Physical Exertion; Potassium

Topics: Animals; Asphyxia Neonatorum; Carbohydrates; Female; Fetal Death; Glycogen; Heart Arrest; Humans; Hyperkalemia; Hypoxia; Infant, Newborn; Myocardium; Pregnancy; Rabbits

Topics: Adenosine Triphosphatases; Adult; Glycogen; Histocytochemistry; Humans; Hyperkalemia; Male; Microscopy, Electron; Muscles; Myofibrils; Paralysis; Succinate Dehydrogenase